Optical biosensor and method of use

• US 5,194,393 A
• Filed: 11/08/1990
• Issued: 03/16/1993
• Est. Priority Date: 11/21/1989
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics GmbH (Siemens AG)
• Original Assignee: Bayer AG
• Inventors: Mobius, Dietmar, Sommer, Klaus, Rolf, Meinhard, Schopper, Heinrich C., Rosenkranz, Hans J., Wehrmann, Rolf, Kuckert, Eberhard, Siegmund, Hans-Ulrich, Hugl, Herbert, Ohst, Holger
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US07/611,197
• Time in Patent Office: 859 Days
• Field of Search: 435/4, 435/173, 435/291, 435/808, 435/968, 422/57, 422/60, 422/58, 422/82.08, 422/82.05, 422/82.07, 422/82.11, 436/537, 436/172, 436/805, 436/807, 436/525, 436/527, 436/531, 436/800, 250/458.1, 250/459.1, 250/461.1, 250/483.1, 250/486.1
• US Class Current: 436/525
• CPC Class Codes: C07D 277/32 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 277/56 Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
• CPC Class Codes: C07D 311/16 substituted in position 7
• CPC Class Codes: C07D 417/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

The present invention relates to a sensor with a novel construction for a detection method of molecules labelled with fluorescent dye for detecting these dissolved substances or analytes by energy transfer with a simple fluorescence technique and increased sensitivity in the detection as well as versatile use for different tasks and the possibility of reproducible preparation of films bound to solid surfaces.

Claims

1. A process for the detection of analyte molecules in a sample comprising providing a sensor consisting of a) a solid support,

b) a single layer or multi-layer Langmuir-Blodgett (LB) film attached to the surface of said support a),

c) at least one fluorescent dye F₁ which is located in the top layer or in case of a multi-layer Langmuir-Blodgett film, in at least one of the top four layers of the Langmuir-Blodgett film,

d) a first receptor molecule which is capable of specific binding with said analyte molecule and which is bonded covalently or by absorption in or on the topmost layer of the Langmuir-Blodgett film, and

e) exposing said sensor to said sample and a mobil fluorescent dye F₂ whose excitation band overlaps sufficiently for an energy transfer with the emission band of said dye F₁ and which

e1) is covalently bonded to a second receptor molecule which competes with said analyte molecules for specifically binding to said first receptor molecule d) or which

e2) is covalently bonded to a third receptor molecule which is able to bind to a complex composed of the first receptor molecule d) and said analyte molecule,

where the second molecule or the complex of the analyte molecule and the third receptor molecule are initially not bound to the Langmuir-Blodgett film,

and detecting a change in the level of fluorescence energy transfer in response the said exposing step.

2. A sensor for the detection of analyte molecules based on fluorescence energy transfer (Forster transfer) consisting of

a) a solid support,

b) a single layer or multi-layer Langmuir-Blodgett film (LB) attached to the surface of said support a),

c) at least one fluorescent dye F₁ which is located in the top layer or in case of a multi-layer Langmuir-Blodgett film, in at least one of the top four layers of the Langmuir-Blodgett film,

d) a receptor molecule which is capable of specific interaction with a ligand molecule and which is bonded covalently or by adsorption in or on the topmost layer of the Langmuir-Blodgett film.

3. A sensor according to claim 2 wherein the solid support used is composed of glass, quartz glass, Li niobate, zinc selenide, porcelain, semi-conductor materials, a metal, a plastic or a metallized plastic.

4. A sensor according to claim 3, wherein the solid support used is composed of glass, quartz glass, silicon, plastic or a metallized plastic.

5. A sensor according to claim 2, wherein the Langmuir-Blodgett film consists of a polymer.

6. A sensor according to claim 5, wherein said dye F₁ is covalently bonded to the polymer.

7. A sensor according to claim 2, wherein the dye F₁ is spread together with an amphiphilic matrix.

8. A sensor according to claim 2, wherein, in the case of a multi-layer Langmuir-Blodgett film the dye F₁ is located in at least one of the two upper layers of the LB film.

9. A sensor according to claim 2, wherein the receptor molecule is attached covalently to the topmost layer of the LB film.

10. The sensor of claim 9, wherein the receptor molecule d) is attached covalently via a spacer molecule to the topmost layer of the LB film.

11. The sensor of claim 2, wherein the receptor molecule d) is located onto the topmost layer of the film via a hydrophobic membrane anchor.

12. Sensor of claim 7 is which the dye F₁ forms Scheibe-aggregates.