Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end

• US 5,196,305 A
• Filed: 09/12/1989
• Issued: 03/23/1993
• Est. Priority Date: 09/12/1989
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Bergmeyer, Lynn, Findlay, John B.
• Primary Examiner(s): Moskowitz, Margaret
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US07/406,221
• Time in Patent Office: 1,288 Days
• Field of Search: 435/6, 435/91, 435/805, 435/948, 436/501, 436/811, 536/27, 935/6, 935/17, 935/19, 935/78, 935/88
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/948 using viruses or cell lines
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function

Abstract

Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3'"'"' end of the primer. Such a mismatch is overcome using a primer having a nucleotide with a thymine base at the position of the mismatch. The use of such primers is most likely to prime the target and form primer extension products. This method is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient samples.

Claims

1. A method for amplifying at least one nucleic acid, said method comprising:

A. contacting a specimen comprising a nucleic acid with a primer composition,

said primer composition consisting essentially of a primer which is complementary to a sequence of said nucleic acid in every position of said sequence except a single position at the 3'"'"' end of said primer, resulting in a single mismatch at said single position between said primer and said nucleic acid sequence,

said primer having a nucleotide with a thymine base in the position of said mismatch, and

B. substantially simultaneously, contacting said specimen with a DNA polymerase under conditions such that said nucleic acid is amplified in the resulting mixture by forming primer extension products, and denaturation of said primer extension products, said product formation and denaturation being carried out at least five times.

2. The method of claim 1 for the amplification of viral DNA.

3. The method of claim 2 for the amplification of HIV-I-DNA.

15. The method of claim 1 wherein said polymerase is isolated from Thermus aquaticus or is prepared using recombinant means.

4. A method for the detection of a target nucleic acid, said method comprising:

A. contacting a specimen comprising a nucleic acid with a primer composition,

said primer composition consisting essentially of a primer which is complementary to a sequence of said nucleic acid in every position of said sequence except a single position at the 3'"'"' end of said primer, resulting in a single mismatch at the single position between said primer and said nucleic acid sequence,

said primer having a nucleotide with a thymine base in the position of said mismatch,

so as to form hybridized products of said primer and said nucleic acid,

B. forming primer extension products in said hybridized products, priming, extending and amplifying said primer extension products using a DNA polymerase in a polymerase chain reaction,

C. separating the resulting primer extension products and contacting them with a detection or capture oligonucleotide probe to form a complementary product, and

D. detecting the presence of said complementary product as an indication of the presence of said target nucleic acid in said specimen.

5. The method of claim 4 using a capture oligonucleotide and detection is accomplished using a biotinylated primer and an avidin-enzyme conjugate.

6. The method of claim 5 wherein said conjugate comprises peroxidase.

7. The method of claim 6 for the detection of HIV-I DNA.

8. The method of claim 4 wherein detection is accomplished using a probe which is labeled or capable of being labeled with an enzyme.

9. The method of claim 8 wherein said enzyme is peroxidase, and said detection is carried out using a composition which provides a dye in the presence of peroxidase and hydrogen peroxidase.

10. The method of claim 4 wherein one of said primers is either attached to or capable of being attached to an insoluble substrate.

16. The method of claim 6 wherein said polymerase is thermostable and is isolated from Thermus aquaticus or is prepared using recombinant means.

11. A method for the detection of at least one predetermined nucleic acid having two complementary strands comprises:

A. denaturing the complementary strands of a double-stranded nucleic acid in a biological specimen,

B. contacting said specimen with a primer composition, said primer composition consisting essentially of a primer which is complementary to a sequence of one of said nucleic acid strands in every position of said sequence except a single position at the 3'"'"' end of said primer, resulting in a single mismatch at said single position between said primer and said nucleic acid strand, said primer having a nucleotide with a thymine base in the position of said mismatch,

said specimen also simultaneously contacted with one or more additional primers which are substantially complementary to the other strand of said nucleic acid,

so as to form hybridized products of said primers and said nucleic acid strands,

C. in the presence of a thermostable polymerase, and the deoxyribonucleotide triphosphates dATP, dCTP, dGTP and dTTP, forming extension products of said primers in said hybridized products, which extension products, when separated from their complements, can serve as templates for synthesis of said extension products of said primers,

D. separating said primer extension products from the templates on which they were synthesized,

E. contacting said separated extension products and said predetermined nucleic acid with additional primers complementary to the strands, resulting in amplification of said nucleic acid to form complementary products,

F. separating said primer extension products from the complementary products formed in step E,

G. contacting at least one primer extension product separated in step F with an oligonucleotide probe which is labeled for detection or capture and is complementary thereto to form a complementary product of the probe and said primer extension product, and

H. detecting the complementary product formed in step G as an indication of the presence of said predetermined nucleic acid in the specimen.

12. The method of claim 11 for the detection of HIV-I DNA in whole blood or semen.

13. The method of claim 11 carried out by repeating steps E and F from 5 to 50 times before performing step G.

14. The method of claim 11 wherein more than one nucleic acid forms a single mismatch with its primer.