Enzyme electrode containing an enzyme and a coenzyme immobilized in separate layers of a membrane
First Claim
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1. An enzyme electrode comprising:
- (a) an electrode;
(b) an inner membrane covering the electrode (a), said inner membrane comprising two separate layers comprising an inner layer in which an enzyme is immobilized and an outer layer in which a coenzyme which is cooperative with the enzyme is contained, and(c) an outer membrane covering the inner membrane (b) and consisting essentially of a material which is difficult for the coenzyme to diffuse therein;
whereby the coenzyme in the inner membrane (b) is prevented from dissipation.
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Abstract
An enzyme electrode comprising an enzyme and a coenzyme immobilized in at least one layer is provided. This electrode has an outer membrane preventing the coenzyme from dissipation and so sensitivity and durability can be improved. In an inner membrane containing the enzyme and the coenzyme, a mediator of electron transfer may be added. The enzyme and the coenzyme may be separated in two layers, respectively, in the inner membrane to improve measurement sensitivity.
9 Citations
6 Claims
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1. An enzyme electrode comprising:
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(a) an electrode; (b) an inner membrane covering the electrode (a), said inner membrane comprising two separate layers comprising an inner layer in which an enzyme is immobilized and an outer layer in which a coenzyme which is cooperative with the enzyme is contained, and (c) an outer membrane covering the inner membrane (b) and consisting essentially of a material which is difficult for the coenzyme to diffuse therein;
whereby the coenzyme in the inner membrane (b) is prevented from dissipation. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification