Immunoassays for thyroid hormones using thyroglobulin
First Claim
1. A method for measuring a thyroid hormone in a serum sample which comprises:
- a. incubating a serum sample with a labeled antibody to a thyroid hormone and a composite comprising a thyroglobulin chemically modified by succinic anhydride immobilized on an insoluble carrier material, to form a complexed composite;
b. separating the complexed composite from unbound labeled antibody;
c. measuring the amount of label associated with the complexed composite or associated with the unbound labeled antibody; and
d. relating the measurement of step c. to the amount of the thyroid hormone in the serum sample.
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Abstract
Immunoassays for measuring thyroid hormones such as thyroxine(T4) and triiodothyronine(T3) are carried out by incubating a serum sample with either (a) unlabeled thyroglobulin and a labeled antibody to a thyroid hormone or to thyroxine binding globulin(TBG), or (B) labeled thyrogloublin and an unlabeled antibody to a thyroid hormone. The thyroglobulin may be immobilized on an insoluble carrier and the immobilized thyroglobulin is preferably modified by succinylation with succinic anhydride. Whole thyroglobulin can be used or the thyroglobulin can be fragmented into smaller peptides prior to use provided the peptides contain at least one T3 or T4 residue per peptide. Preferably, the antibody is a monocional antibody and labeling is with an acridinium ester.
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Citations
2 Claims
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1. A method for measuring a thyroid hormone in a serum sample which comprises:
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a. incubating a serum sample with a labeled antibody to a thyroid hormone and a composite comprising a thyroglobulin chemically modified by succinic anhydride immobilized on an insoluble carrier material, to form a complexed composite; b. separating the complexed composite from unbound labeled antibody; c. measuring the amount of label associated with the complexed composite or associated with the unbound labeled antibody; and d. relating the measurement of step c. to the amount of the thyroid hormone in the serum sample.
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2. A method for measuring a thyroid hormone in a serum sample which comprises:
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a. incubating a serum sample with labeled thyroglobulin chemically modified by succinic anhydride and a composite comprising antibody to a thyroid hormone immobilized on an insoluble carrier material, to form a complexed composite; b. separating the complexed composite from unbound labeled thyroglobulin; c. measuring the amount of label associated with the complexed composite or associated with the unbound labeled thyroglobulin; and d. relating the measurement of step c. to the amount of the thyroid hormone in the serum sample.
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Specification