Vectors in use in filamentous fungi
First Claim
1. A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host.
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Reexamination
Accused Products
Abstract
Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
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Citations
17 Claims
- 1. A DNA construct for use in transforming an Aspergillus host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus host.
- 4. A DNA construct for use in transforming an Aspergillus nidulans host to obtain expression therein of a polypeptide foreign thereto, said DNA construct comprising promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene operably linked to DNA coding for said polypeptide to enable expression thereof in said Aspergillus nidulans host.
- 7. An Aspergillus strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
- 10. An Aspergillus nidulans strain which upon culturing expresses a polypeptide foreign thereto as a result of having been transformed by a DNA construct in which DNA coding for the foreign polypeptide is linked operably to promoter DNA corresponding in sequence to the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
- 12. A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus strain is linked operably to promoter DNA corresponding in sequence to the promoter region of the glucoamylase gene of Aspergillus niger.
- 15. A method for producing a polypeptide, said method comprising the step of culturing an Aspergillus nidulans strain transformed by a DNA construct in which DNA coding for a polypeptide foreign to said Aspergillus nidulans is linked operably to promoter DNA corresponding in sequence with the promoter region of an Aspergillus nidulans gene selected from the group consisting of the alcohol dehydrogenase 1 gene and the aldehyde dehydrogenase gene.
Specification