×

Homogeneous amperometric immunoassay

  • US 5,198,367 A
  • Filed: 06/09/1989
  • Issued: 03/30/1993
  • Est. Priority Date: 06/09/1989
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for determining the concentration of an analyte present in a sample of biological fluid contained in an electrochemical cell equipped with a set of electrodes, said method comprising the steps of:

  • a) forming an electroactive complex containing an antigenic analyte covalently bonded to a carrier molecule, said carrier molecule also being covalently bonded to an electroactive molecule capable of transferring a charge to an electrode,b) adding the biological fluid, predetermined amounts of the electroactive complex, and an antibody to the analyte to the cell,c) applying a voltage across the electrodes and measuring the current in the fluid at different voltage levels to form a voltage/current relationship for the sample fluid, andd) comparing the voltage/current relationship for the sample fluid with standard voltage/current relationships generated using known amounts of analytes to determine the concentration of analyte present in the sample.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×