Digoxigenin derivatives and use thereof

• US 5,198,537 A
• Filed: 10/25/1989
• Issued: 03/30/1993
• Est. Priority Date: 10/27/1988
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Huber, Erasmus, Zink, Bruno, von der Eltz, Herbert, Muhlegger, Klaus
• Primary Examiner(s): Bond, Robert T.
• Assistant Examiner(s): WARD, EDWARD C
• Application Number:
  US07/427,249
• Time in Patent Office: 1,252 Days
• Field of Search: 540/104, 540/105, 424/1.1, 536/28, 536/6.1, 536/27, 530/406, 530/345, 530/405, 530/388.24
• US Class Current: 530/406
• CPC Class Codes: C07J 19/00 Normal steroids containing carbon, hydrogen, halogen or oxygen, substituted in position 17 by a lactone ring
• CPC Class Codes: C07J 41/0055 the 17-beta position being substituted by an uninterrupted chain of at least three carbon atoms which may or may not be branched, e.g. cholane or cholestane derivatives, optionally cyclised, e.g. 17-beta-phenyl or 17-beta-furyl derivatives
• CPC Class Codes: C07J 43/003 not condensed
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals

Abstract

The present invention provides digoxigenin derivatives of the formula: ##STR1## wherein n is a whole number of from 1 to 4 and Z is a carboxyl group or a functional derivative derived therefrom. The present invention also provides digoxigenin conjugates of the general formula: ##STR2## wherein the carrier is an immunogenic carrier material, a nucleic acid or a labelled immunocomponent of a labelled digoxigenin immunoconjugate for use in an immunoassay, m is a number from 1 to the number of coupling positions available on the carrier and Y is the group formed by the reaction of the carboxyl function Z of the digoxigenin derivative of general formula (I) with the reacting position of the carrier material. Also described are uses of these conjugates as nucleic acid probes, immunogens, and in immunoassay.

Claims

1. Digoxigenin derivative of formula:

##STR9## wherein n is a whole number from 1 to 4, and Z is --CN, --COOC₂ H₅, ##STR10##

2. Digoxigenin derivative of claim 1, wherein n is 1.

3. Digoxigenin conjugate of the formula:

##STR11## wherein said carrier is an immunogenic protein or polypeptide, a nucleic acid or a labelled immunocomponent of a labelled digoxigenin immunoconjugate, m is a whole number from 1 to the number of coupling positions available on said carrier, and Y is ##STR12##

4. Digoxigenin conjugate of claim 3, wherein said carrier is an immunogenic protein or polypeptide.

5. Digoxigenin conjugate of claim 3, wherein said carrier is a nucleic acid.

6. Digoxigenin conjugate of claim 3, wherein said carrier is a labelled immunocomponent.