Contour mapping of spectral diagnostics

• US 5,201,318 A
• Filed: 10/04/1991
• Issued: 04/13/1993
• Est. Priority Date: 04/24/1989
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Rava, Richard P., Richards-Kortum, Rebecca, Feld, Michael S., Baraga, Joseph J.
• Primary Examiner(s): Kamm, William E.
• Assistant Examiner(s): Pontius, Kevin
• Application Number:
  US07/772,620
• Time in Patent Office: 557 Days
• Field of Search: 128/633, 128/664, 128/665
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

The present invention relates to a method of generating and processing spectral information arising from the laser induced fluorescence of tissue. The intensity of fluorescence is recorded as a function of both excitation and emission wavelengths and contour maps of tissue fluorescence are generated which are useful in the diagnosis of condition of the tissue under examination.

Claims

1. A method of processing spectra generated from the induced fluorescence of tissue comprising:

irradiating a portion of tissue over a range of periodically separated excitation wavelengths with radiation from a light source to induce autofluorescence of the tissue such that the tissue emits fluorescent radiation at a multiplicity of emission wavelengths;

forming a spectrum of the intensity of the emitted radiation from the recorded fluorescing radiation at each of the multiplicity of excitation and emission wavelengths;

repeating the irradiating and forming steps to provide a plurality of spectra;

averaging the plurality of spectra to produce an average spectrum; and

identifying fluorophores present in the tissue that have predetermined fluorescence characteristics which correlate with spectral features in the average spectrum, the feature having identified excitation and emission wavelengths present in the average spectrum.

2. The method of processing spectra of claim 1 wherein the tissue comprises arterial tissue.

3. The method of processing spectra of claim 1 wherein the tissue comprises colon tissue.

4. The method of processing spectra of claim 1 wherein the tissue comprises bladder tissue.

5. The method of processing spectra of claim 1 further comprising the step of forming a difference spectra by taking the difference between the formed spectrum and a reference spectrum.

6. The method of processing spectra of claim 1 further comprising identifying a region within the spectrum wherein the intensity in the region is reduced by a component of tissue which absorbs radiation.

7. The method of processing spectra of claim 6 further comprising adjusting the intensity in the region to correct for a contribution to the spectrum by the absorbing component of tissue.

8. The method of processing spectra of claim 1 further comprising the step of forming a ratio spectrum by dividing the formed spectrum by a reference spectrum.

9. A method of diagnosing bodily tissue comprising:

inserting a catheter containing an optical fiber into a body lumen and positioning a distal end of the catheter adjacent to tissue to be diagnosed;

coupling a proximal end of the catheter to a radiation source such that the radiation is transmitted along the optical fiber and directed onto the tissue to be diagnosed;

inducting autofluorescence with the tissue with the radiation source with a multiplicity of periodically separated excitation wavelengths;

recording the autofluorescent radiation emitted by the tissue at a multiplicity of emission wavelengths;

forming a spectral matrix of the intensity of the emitted radiation from the recorded radiation at each of the multiplicity of excitation and emission wavelengths;

repeating the inducing and recording steps to provide a plurality of the spectral matrices;

averaging the plurality of spectral matrix to produce an average spectral matrices; and

identifying fluorophores present in the tissue from the average spectral matrix to diagnose a condition of the tissue.

10. The method of claim 9 further comprising comparing the spectral matrix with a reference matrix to determine the presence of abnormal tissue.

11. The method of claim 9 wherein the radiation source comprises a laser.

12. The method of claim 9 wherein the lumen comprises an artery such that fluorophores of atherosclerotic material within the artery are identified.

13. The method of claim 9 further comprising identifying a portion of the spectral matrix wherein the intensity of the emitted radiation is reduced by a component of tissue which absorbs radiation.

14. The method of claim 13 further comprising adjusting the intensity of the identified portion of the spectral matrix to correct for the reduced intensity.

15. A method of diagnosing vascular tissue comprising:

inserting a catheter containing an optical fiber into a vascular lumen and positioning a distal end of the catheter adjacent to vascular tissue to be diagnosed;

coupling a proximal end of the catheter to a laser radiation source such that the laser radiation is transmitted along the optical fiber and directed onto the tissue to be diagnosed;

inducing inelastically scattered light to be emitted from the vascular tissue with the laser radiation with a multiplicity of periodically separated excitation wavelengths;

recording the scattered radiation returning from the tissue at a multiplicity of emission wavelengths;

forming a spectral matrix of the intensity of the emitted radiation from the recorded radiation at each of the multiplicity of excitation and emission wavelengths;

repeating the inducing and recording steps to provide a plurality of spectral matrices;

averaging the spectral matrices to provide an average spectral matrix; and

identifying abnormal tissue present in the tissue from the average spectral matrix.

16. The method of claim 15 further comprising comparing the spectral matrix with a reference matrix to determine the presence of abnormal tissue.

17. The method of claim 15 wherein the lumen comprises an artery such that fluorophores of atherosclerotic material within the artery are identified.

18. The method of claim 15 further comprising identifying a portion of the spectral matrix wherein the intensity of the emitted radiation is reduced by a component of tissue which absorbs radiation.

19. The method of claim 18 further comprising adjusting the intensity of the identified portion of the spectral matrix to correct for the reduced intensity.

20. The method of claim 15 wherein the inelastically scattered light comprises fluorescence.