Evacuating apparatus for a microtitration diaphragm plate

• US 5,201,348 A
• Filed: 02/27/1992
• Issued: 04/13/1993
• Est. Priority Date: 03/07/1991
• Status: Expired due to Term

• Current Assignee: Eppendorf AG
• Original Assignee: Eppendorf-Netheler-Hinz GmbH (Eppendorf Se)
• Inventors: Lurz, Werner
• Primary Examiner(s): Recla, Henry J.
• Assistant Examiner(s): Douglas, Steven O.
• Application Number:
  US07/843,907
• Time in Patent Office: 411 Days
• Field of Search: 141/130, 141/67, 141/69, 141/7, 141/65, 141/59, 422/100, 422/101, 222/152, 73/864.91, 210/416.1
• US Class Current: 141/130
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples

Abstract

An evacuating apparatus for applying suction to the underside of a microtitration diaphragm plate including a plurality of diaphragm receptacles each having an open top and a diaphragm bottom, the evacuating apparatus including a housing defining an evacuation chamber having an aperture provided in a top wall of said chamber and having associated therewith peripheral gasket means for supporting a rim of said microtitration diaphragm plate, and further comprising a carrier including support means for supporting a microtitration recipient plate including a plurality of closed bottom receptacles each having an open top and a closed bottom, said carrier being adapted to be displaced between an access position wherein said recipient plate may be positioned onto or removed from said support means, and a loading position wherein said recipient plate is disposed within said chamber below said diaphragm plate so that said diaphragm receptacles of said diaphragm plate vertically register with said closed bottom receptacles of said recipient plate, said carrier having a face plate which sealingly engages a peripheral edge of an access opening of said chamber when said carrier is in its loading position.

Claims

1. An evacuating apparatus for applying suction to the underside of a microtitration diaphragm plate including a plurality of diaphragm receptacles each having an open top and a diaphragm bottom, the evacuating apparatus including a housing (1) defining an evacuation chamber (2) having an aperture (4) provided in a top wall of said chamber (2) and having associated therewith peripheral gasket means (5) for supporting a rim of said microtitration diaphragm plate (6), and further comprising a carrier (18) including support means (8'"'"') for supporting a microtitration recipient plate (19) including a plurality of closed bottom receptacles (20) each having an open top and a closed bottom, said carrier (18) being adapted to be displaced between an access position wherein said recipient plate (19) may be positioned onto or removed from said support means (8'"'"'), and a loading position wherein said recipient plate (19) is disposed within said chamber (2) below said diaphragm plate (6) so that said diaphragm receptacles (7) of said diaphragm plate (6) vertically register with said closed bottom receptacles (20) of said recipient plate (19), said carrier (18) having a face plate (15) which sealingly engages a peripheral edge of an access opening (30) of said chamber (2) when said carrier (18) is in its loading position.

2. An evacuation apparatus as defined in claim 1 which includes a plurality of apertures (4) for receiving a plurality of diaphragm plates (6) and a plurality of associated chambers (2) for a plurality of recipient plates (19).

3. An evacuation apparatus as defined in claim 2 wherein said plurality of evacuation chambers (2) are adapted to be connected to a vacuum pump (13) by valve switch means.

4. An evacuation apparatus as defined in any of claims 1 to 3, wherein said gasket means comprises a gasket member (5) having an upstanding leg portion for engagement with said diaphragm plate (6).

5. An evacuation apparatus as defined in any of claims 1 to 4 including an urging plate (9) adapted to be pivoted between a release position allowing to position or remove said diaphragm plate (6) onto or from said gasket means (5) and an operative position wherein said urging plate (9) urges said diaphragm plate (6) downwards into engagement with said gasket means (5).

6. An evacuation apparatus as defined in claim 5 including switch means for energizing said vacuum pump (13) connected to said evacuation chamber (2), said urging plate (9) being adapted to actuate said switch means when the urging plate (9) is in its operative position.

7. An evacuation apparatus as defined in any of claims 1 to 5, wherein said carrier (18) is displaceable in a direction parallel to said diaphragm plate (6).

8. An evacuation apparatus as defined in any of claims 1 to 7, wherein said carrier (18) is provided on a drawer (14) or a table pivotal in a plane parallel to said diaphragm plate (6) or on a flap pivotal in a plane perpendicular to said diaphragm plate (6).

9. An evacuation apparatus as defined in any of claims 1 to 8, wherein said support means (8,) for said recipient plate (19) is carried by said carrier (18) releasably secured to said face plate (15).

10. An evacuation apparatus as defined in any of claims 1 to 9, wherein said carrier (18) has associated therewith a guide element (25) or a pivoting element connected to a said face plate (15), said guide elementor pivoting element including lineal guide means (26) of pivotal guide means outside of said chamber (2).

11. An evacuation apparatus as defined in claim 10, wherein said guide element (25) or pivoting element is connected to a face plate support (23) by resilient means (22) allowing for relative movements between said face plate support (23) and said face plate (15) in the direction of guiding movements as enabled by said guide element or pivoting element.

12. An evacuation apparatus as defined in any of claims 1 to 11, wherein said face plate (15) includes gasket means (29) adapted to engage said housing(1) for sealingly closing said access opening (30) of said chamber (2).

13. An evacuation apparatus as defined in any of claims 1 to 12 including a pan (8) adapted to be inserted into said aperture (4) in said top wall of said chamber (2) or adapted to be disposed on said carrier (18), said pan (8) having an outwardly extending peripheral flange comprising said support means (8'"'"'), which peripheral flange (8'"'"') engages said gasket means (5) of said aperture (4) when said pan (8) is inserted into said aperture (4).

14. An evacuation apparatus as defined in any of claims 1 to 13, wherein said carrier (18) is adapted to be locked when it is in its loading position.

15. An evacuation apparatus as defined in claim 14, wherein said carrier (18) has associated therewith a locking lever having an actuating leg extending through said face plate (15) or said face plate support (23) and a locking leg engaging a locking pin when the locking lever is in a locking position.

16. An evacuation apparatus as defined in claim 12 or claim 13, wherein said carrier is arranged to bias a return spring when it is moved towards its loading position.

18. An evacuation apparatus as defined in any of claims 1 to 17, wherein a further chamber (3) is provided within said housing (1), which further chamber (3) includes said vacuum pump (13), electrical control and supply means (34, 33), said guide element (26) or pivoting element, locking means, a return spring and/or a displacement motor (28).

19. An evacuation apparatus as defined in any of claims 1 to 18, wherein a sterilized filter (32) is disposed in said evacuation chamber (2) and is connected to said vacuum pump (13) by a connection line sealingly extending through a wall of said evacuation chamber (2).

20. An evacuation apparatus as defined in any of claims 1 to 17 including rubber feet preferrably comprising suction cups (35).

17. An evacuation apparatus as defined in any of claims to 14, wherein said carrier (18) is associated with said guide element (25) extending in the direction of displacement and being in engagement with a pinion (27) of a displacement motor (28).