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Production of chimeric antibodies by homologous recombination

  • US 5,204,244 A
  • Filed: 09/14/1988
  • Issued: 04/20/1993
  • Est. Priority Date: 10/27/1987
  • Status: Expired due to Term
First Claim
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1. A method for the production of a cell line expressing a modified antibody molecule comprising:

  • (a) transfecting an antibody producing lymphoid cell line with a target vector comprising;

    (i) a replacement gene to modify a portion of the genomic sequence of the immunoglobulin genes of the lymphoid cell line, and(ii) a target sequence homologous to a second DNA sequence adjacent to the immunoglobulin sequence to be converted, so that the replacement gene modifies the immunoglobulin sequence via site specific homologous recombination with genomic chromosomal DNA in vivo; and

    (b) selecting a transfectant which produces the modified antibody molecule.

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