Colorimetric assay by enzymatic oxidation in the presence of an aromatic nitroso or oxime compound
First Claim
1. A process for the colorimetric determination of an analyte which comprises:
- a) oxidizing the analyte in a reaction mixture comprising an oxidoreductase enzyme which is dependent from the cofactors flavin or PQQ, said enzyme catalyzing said oxidizing of the analyte, and a color-forming aromatic nitroso compound or a tautomerically equivalent oxime compound which directly accepts electrons from the enzyme/cofactor and causes a color change in the reaction mixture;
b) measuring the color change in the reaction mixture; and
c) comprising standard values to the color change to obtain the amount of the analyte being oxidized.
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Abstract
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of the reduced electron acceptor by color formation as measure for the amount of the analyte, wherein the analyte is oxidized with an appropriate flavine- or PQQ-dependent oxidoreductase in the presence of aromatic nitroso or oxime compounds. The present invention also provides an agent for the colorimetric determination of an analyte by enzymatic oxidation of the analyte, containing flavine- or PQQ-dependent oxidoreductase and a color-forming electron acceptor, wherein the color-forming electron acceptor is an aromatic nitroso or oxime compound.
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Citations
8 Claims
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1. A process for the colorimetric determination of an analyte which comprises:
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a) oxidizing the analyte in a reaction mixture comprising an oxidoreductase enzyme which is dependent from the cofactors flavin or PQQ, said enzyme catalyzing said oxidizing of the analyte, and a color-forming aromatic nitroso compound or a tautomerically equivalent oxime compound which directly accepts electrons from the enzyme/cofactor and causes a color change in the reaction mixture; b) measuring the color change in the reaction mixture; and c) comprising standard values to the color change to obtain the amount of the analyte being oxidized. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification