Assembly for front face testing

• US 5,208,649 A
• Filed: 02/20/1992
• Issued: 05/04/1993
• Est. Priority Date: 02/20/1992
• Status: Expired due to Fees

• Current Assignee: University of Cincinnati
• Original Assignee: University of Cincinnati
• Inventors: Cuppoletti, John, Langdon, Nick H.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/839,469
• Time in Patent Office: 439 Days
• Field of Search: 356/244, 356/317, 356/318, 250/458.1, 250/461.1, 250/461.2
• US Class Current: 356/244
• CPC Class Codes: G01N 2021/0367 Supports of cells, e.g. pivotable
• CPC Class Codes: G01N 2021/6476 Front end, i.e. backscatter, geometry
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation

Abstract

A coverslip holder assembly for a front face fluorimetry flow cell has a holder body and a hingedly attached clamp for releasably maintaining a coverslip adjacent the front face of the holder body and flow cell. The assembly is preferably a unitary arrangement wherein the clamp is hingedly mounted adjacent the bottom end of the front face of the holder body, and has a convenient snap lock release mechanism at its top end to selectively lock the coverslip in flush arrangement with the front face. The holder assembly can provide a biased snug fit with a variety of cuvettes or similar sample vessels, and enables the use of highly colored or fluorescent compounds for perfusion in front face fluorimetry operations.

Claims

1. A holder assembly for a coverslip, said holder adapted for use in conjunction with a sample vessel and a device for assaying the light absorbing, reflecting or other optical characteristics of substances on such coverslip, and

a holder body having spaced front and rear faces, and top and bottom ends, with said front face having a substantially planar portion, means located adjacent the bottom end of said front face for supporting a coverslip substantially flush with said planar portion, and a non-reflecting light sink;

and

a clamp releasably attachable to said holder body, said clamp comprising means for maintaining a coverslip in substantially flush supported position relative to said front face of said holder body, and a light permeable portion generally aligned with said planar portion when in attached position with said holder body.

2. The holder assembly of claim 1, further comprising means for hingedly attaching said clamp to said holder body, whereby said clamp may be articulated between open and attached positions.

3. The holder assembly of claim 2, wherein said clamp further comprises a hinged connection with said holder body adjacent one of said top and bottom ends, and means for locking said clamp in attached position with said holder body.

4. The holder assembly of claim 1, wherein said holder body is provided as a unitary structure having means for biasing said holder assembly outwardly against a sample vessel.

5. The holder assembly of claim 4, wherein said sample vessel is a cuvette, and wherein said biasing means comprises at least one compressible portion formed within said holder body.

6. The holder assembly of claim 1, further comprising means for enabling perfusion of cells on said coverslip while said coverslip is held within said assembly.

7. The holder assembly of claim 1, wherein said clamp comprises an inwardly facing portion having at least one support protuberance which extends inwardly to resiliently secure a coverslip between said holder body and said clamp in attached position.

8. The holder assembly of claim 2, wherein said clamp further comprises a hinged connection with said holder body adjacent one of said top and bottom ends, and snap fit means for releasably locking said clamp in attached position with said holder body.

9. The holder assembly of claim 1, wherein said holder body and said clamp provide an exterior conformation which substantially corresponds to the inner conformation of a sample vessel when in attached position.

10. An assembly for holding a coverslip upon which cells may be cultured for study within a sample vessel and device for assaying the light absorbing, reflecting or optical characteristics of such cells, said holder comprising:

a holder body having spaced front and rear faces, top and bottom ends, and a nonreflecting light path between said front and rear surfaces, said front face having a substantially planar portion and means located adjacent the bottom end of said front face for supporting a coverslip substantially flush with said planar portion; and

a clamp which is hingedly attached to said holder body, said clamp comprising means for maintaining a coverslip in substantially flush supported position relative to said front face of said holder body and a light permeable portion aligned with said light path when in attached position with said holder body, said clamp being rotatable away from said front face to facilitate coverslip loading, unloading and cleaning procedures.

11. The assembly of claim 10, wherein said clamp is hingedly attached to said holder body adjacent one of said top and bottom ends, and further comprising means for locking said clamp in attached position with said holder body.

12. The assembly of claim 11, wherein said locking means comprises an interacting snap lock and corresponding groove arrangement adjacent the top ends of said holder body and clamp, respectively.

13. The holder assembly of claim 10, wherein said holder body is provided as a unitary structure having means for biasing said holder assembly outwardly against the inside of a sample vessel.

14. The holder assembly of claim 13, wherein said sample vessel is a cuvette, and wherein said biasing means comprises at least one spring slot formed in said holder body.

15. The holder assembly of claim 10, further comprising means for enabling perfusion of cells on said coverslip while said coverslip is held within said assembly.

16. The holder assembly of claim 10, wherein said clamp comprises an inwardly facing portion having at least one support protuberance extending to resiliently secure a coverslip between said holder body and said clamp in attached position.

17. An assembly for holding a coverslip upon which cells may be cultured for study within a cuvette and device for front face measurement of fluorescence of such cells, said holder comprising:

a holder body having spaced front and rear faces, top and bottom ends, and a relatively hollow light path extending between said front and rear surfaces, said front face having a substantially planar portion and means located adjacent the bottom end of said front face for supporting a coverslip substantially flush with said planar portion;

a clamp which is hingedly attached to said holder body, said clamp comprising a light permeable sight path substantially aligned with said light path when in attached position with said holder body; and

means for selectively locking said clamp in attached position adjacent said front face of said holder body, wherein said coverslip is held in substantially flush supported position relative to said front face and aligned with said sight path.

18. The assembly of claim 17, wherein said holder body is provided as a unitary structure having means for biasing said assembly outwardly against the inside of a cuvette with said front face and a supported coverslide located closely adjacent and normal to the incident light of said device for measuring the fluorescence of said cells on the coverslide.

19. The holder assembly of claim 17, further comprising means for enabling perfusion of cells on said coverslip while said coverslip is held within said assembly.

20. The holder assembly of claim 18, wherein said sample vessel is a cuvette, and wherein said biasing means comprises at least one spring slot formed in said holder body.