Bufferless electrophoresis system and method
First Claim
Patent Images
1. A disposable electrophoresis apparatus comprising:
- a housing having opposed top, bottom and side walls defining a three dimensional chamber having opposed open ends;
a three dimensional body of gel disposed in said chamber and exposed at said open ends;
means in said top wall for introducing a sample into said body of gel;
an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and
vent means at said ends.
0 Assignments
0 Petitions
Accused Products
Abstract
A bufferless electrophoresis system for separation of DNA or the like includes a disposable cassette comprising a housing having open ends, a three dimensional gel body in the housing for defining a path, a sample well in a top surface of the gel for introducing a sample into the path at one end thereof, an electrically conductive film closing the ends of the housing and contacting the ends of the gel, and an electrical circuit for selectively applying an electrical potential via the electrically conductive film to the ends of the gel. A holder having spaced apart opposed terminals may be used to hold the cassette and apply the electrical circuit to the cassette.
-
Citations
20 Claims
-
1. A disposable electrophoresis apparatus comprising:
-
a housing having opposed top, bottom and side walls defining a three dimensional chamber having opposed open ends; a three dimensional body of gel disposed in said chamber and exposed at said open ends; means in said top wall for introducing a sample into said body of gel; an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and vent means at said ends. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A portable apparatus for the electrophoresis separation of DNA and the like, said apparatus comprising:
-
a housing having opposed top, bottom and side walls, and a plurality of partition walls defining a plurality of elongated parallel three dimensional chambers having opposed open ends; a three dimensional body of gel disposed in each of said chambers and exposed at said open ends; means including an opening through said top wall and a cavity formed in each of said body of gel for introducing a sample therein; an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and a plurality of vent holes in said top wall at said ends. - View Dependent Claims (13, 14, 15, 16)
-
-
17. A portable apparatus for the electrophoresis separation of DNA and the like, said apparatus comprising:
-
a generally rectangular box-like housing having opposed top, bottom and side walls, and a plurality of partition walls defining a plurality of elongated parallel three dimensional chambers having opposed open ends; a three dimensional body of gel at least 0.05 inches in thickness disposed in each of said chambers and exposed at said open ends; means including an opening through said top wall and a cavity formed in each of said bodies of gel for introducing a sample therein; an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and a plurality of vent holes in said top wall at said ends. - View Dependent Claims (18, 19, 20)
-
Specification