Bufferless electrophoresis system and method

• US 5,209,831 A
• Filed: 06/14/1991
• Issued: 05/11/1993
• Est. Priority Date: 06/14/1991
• Status: Expired due to Fees

• Current Assignee: MacConnell, William P.
• Original Assignee: MacConnell, William P.
• Inventors: MacConnell, William P.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Koestner, Caroline
• Application Number:
  US07/715,392
• Time in Patent Office: 697 Days
• Field of Search: 204/299 R
• US Class Current: 204/616
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44756 Apparatus specially adapted therefor

Abstract

A bufferless electrophoresis system for separation of DNA or the like includes a disposable cassette comprising a housing having open ends, a three dimensional gel body in the housing for defining a path, a sample well in a top surface of the gel for introducing a sample into the path at one end thereof, an electrically conductive film closing the ends of the housing and contacting the ends of the gel, and an electrical circuit for selectively applying an electrical potential via the electrically conductive film to the ends of the gel. A holder having spaced apart opposed terminals may be used to hold the cassette and apply the electrical circuit to the cassette.

Claims

1. A disposable electrophoresis apparatus comprising:

a housing having opposed top, bottom and side walls defining a three dimensional chamber having opposed open ends;

a three dimensional body of gel disposed in said chamber and exposed at said open ends;

means in said top wall for introducing a sample into said body of gel;

an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and

vent means at said ends.

2. A electrophoresis apparatus according to claim 1 wherein said gel is at least 0.05 inches in thickness.

3. A electrophoresis apparatus according to claim 1 wherein said means for introducing a sample comprises a opening through said top wall and a cavity formed in said gel.

4. A electrophoresis apparatus according to claim 1 wherein said vent means comprises a plurality of holes in said top wall at the ends thereof.

5. A electrophoresis apparatus according to claim 4 wherein said vent holes are covered until said unit is ready for use.

6. A electrophoresis apparatus according to claim 1 wherein said housing has a plurality of partition walls defining a plurality of parallel three dimensional chambers having opposed open ends.

7. A electrophoresis apparatus according to claim 1 wherein said housing is at least three inches in length.

8. A electrophoresis apparatus according to claim 7 wherein at least said top wall is transparent.

9. A electrophoresis apparatus according to claim 1 further comprising a holder having opposed spaced apart terminals for conductively engaging said electrically conductive thin film for applying an electrical voltage across the length of said gel.

10. A electrophoresis apparatus according to claim 1 wherein said electrically conductive thin film extends over and is secured to a portion of said top and bottom walls along the end thereof.

11. A electrophoresis apparatus according to claim 10 wherein said electrically conductive thin film is secured to said top and bottom walls by means of double stick tape and sealingly covers said vent means.

12. A portable apparatus for the electrophoresis separation of DNA and the like, said apparatus comprising:

a housing having opposed top, bottom and side walls, and a plurality of partition walls defining a plurality of elongated parallel three dimensional chambers having opposed open ends;

a three dimensional body of gel disposed in each of said chambers and exposed at said open ends;

means including an opening through said top wall and a cavity formed in each of said body of gel for introducing a sample therein;

an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and

a plurality of vent holes in said top wall at said ends.

13. A electrophoresis apparatus according to claim 12 wherein said gel is at least 0.05 inches in thickness.

14. A electrophoresis apparatus according to claim 13 wherein said electrically conductive thin film extends over and is secured to a portion of said top and bottom walls along the end thereof.

15. A electrophoresis apparatus according to claim 13 wherein said electrically conductive thin film is secured to said top and bottom walls by means of double stick tape and sealingly covers said vent means.

16. A electrophoresis apparatus according to claim 12 further comprising a holder having opposed spaced apart terminals for conductively engaging said electrically conductive thin film for applying an electrical voltage across the length of said gel.

17. A portable apparatus for the electrophoresis separation of DNA and the like, said apparatus comprising:

a generally rectangular box-like housing having opposed top, bottom and side walls, and a plurality of partition walls defining a plurality of elongated parallel three dimensional chambers having opposed open ends;

a three dimensional body of gel at least 0.05 inches in thickness disposed in each of said chambers and exposed at said open ends;

means including an opening through said top wall and a cavity formed in each of said bodies of gel for introducing a sample therein;

an electrically conductive thin film disposed in conductive contact with each end of said body of gel and sealingly covering said open ends; and

a plurality of vent holes in said top wall at said ends.

18. A electrophoresis apparatus according to claim 17 wherein said electrically conductive thin film overlaps and is secured to a portion of said top and bottom walls along the end thereof and sealingly covers said vent holes.

19. A electrophoresis apparatus according to claim 17 wherein said said electrically conductive thin film is secured to said top and bottom walls by means of double stick tape.

20. A electrophoresis apparatus according to claim 19 further comprising a generally rectangular open top holder having opposed spaced apart elongated bar terminals for engaging across the ends of said housing and conductively engaging said electrically conductive thin film for applying an electrical voltage across the length of said gel.