Agglutination reaction device utilizing selectively impregnated porous material
First Claim
1. A device for performing agglutination reactions comprising:
- in adherent relationship, a first hydrophilic layer, a second layer parallel to and overlying said first layer, and a third layer parallel to and overlying said second layer and having a window for observing agglutinated particles, said second layer interposed between and in adherent relationship to said first and third layers, said second layer comprising a liquid-absorbent material selectively impregnated with a substance to form an impregnated region and a non-impregnated region wherein said non-impregnated region is liquid-absorbent and said impregnated region is liquid-occlusive, said second layer including means defining a slot in said impregnated region and defining a channel for directing liquid conducted by capillary action through a chamber defined by said slot in conjunction with said first and third layers and within which chamber agglutination reactions can be performed, said chamber having a proximate zone and a distal zone and means for controlling overall rate of liquid flow through said chamber, said means for controlling comprising said non-impregnated region of said second layer which non-impregnated region is in liquid communication with said chamber and wherein a portion of the non-impregnated region is positioned within the distal zone of said chamber.
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Abstract
An improved device for performing agglutination reactions is disclosed. The device has at least one chamber with a proximate zone and a distal zone in which chamber agglutination reactions can be performed. The improvement comprises utilization in the device of a layer of liquid absorbent material for controlling overall rate of liquid flow through the chamber. The layer of liquid absorbent material is selectively impregnated with a substance to form an impregnated region and a non-impregnated region wherein the non-impregnated region is liquid absorbent and the impregnated region is liquid-occlusive. A portion of the non-impregnated region is positioned within the distal zone of the chamber so that the non-impregnated region is in liquid communication with that zone.
116 Citations
20 Claims
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1. A device for performing agglutination reactions comprising:
- in adherent relationship, a first hydrophilic layer, a second layer parallel to and overlying said first layer, and a third layer parallel to and overlying said second layer and having a window for observing agglutinated particles, said second layer interposed between and in adherent relationship to said first and third layers, said second layer comprising a liquid-absorbent material selectively impregnated with a substance to form an impregnated region and a non-impregnated region wherein said non-impregnated region is liquid-absorbent and said impregnated region is liquid-occlusive, said second layer including means defining a slot in said impregnated region and defining a channel for directing liquid conducted by capillary action through a chamber defined by said slot in conjunction with said first and third layers and within which chamber agglutination reactions can be performed, said chamber having a proximate zone and a distal zone and means for controlling overall rate of liquid flow through said chamber, said means for controlling comprising said non-impregnated region of said second layer which non-impregnated region is in liquid communication with said chamber and wherein a portion of the non-impregnated region is positioned within the distal zone of said chamber.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A device for performing simultaneously a plurality of agglutination reactions comprising:
- in adherent relationship, a first hydrophilic layer, a second layer parallel to and overlying said first layer, and a third layer parallel to and overlying said second layer and having windows for observing agglutinated particles, said second layer interposed between and in adherent relationship to said first and third layers, said second layer comprising a liquid-absorbent material selectively impregnated with a substance to form an impregnated region and a plurality of non-impregnated regions wherein said non-impregnated regions are liquid-absorbent and said impregnated region is liquid-occlusive, said second layer including means defining a plurality of slots in radial spatial relationship to each other in said impregnated region and defining channels for directing liquid conducted by capillary action through chambers defined by said slots in conjunction with said first and third layers and within which chambers agglutination reactions can be performed simultaneously, each of said chambers having a proximate zone and a distal zone and means for controlling overall rate of liquid flow through said chamber, said means for controlling comprising a non-impregnated region of said second layer which non-impregnated region is in liquid communication with said chamber and wherein a portion of the non-impregnated impregnated region is positioned within the distal zone of said chamber.
- View Dependent Claims (15, 16)
- 17. An improved device for performing agglutination reactions having at least one chamber having a proximate zone and a distal zone in which chamber agglutination reactions can be performed, the improvement comprising utilization in said device of means for controlling overall rate of liquid flow through said chamber, said means for controlling comprising a layer of liquid-absorbent material selectively impregnated through a thickness thereof with a substance to form an impregnated region and a non-impregnated region wherein said non-impregnated region is liquid-absorbent and said impregnated region is liquid-occlusive and wherein a portion of said non-impregnated region is positioned within said distal zone of said chamber so that said non-impregnated region is in liquid communication with said distal zone of said chamber.
Specification