Test carrier for the determination of ions

• US 5,211,914 A
• Filed: 05/10/1991
• Issued: 05/18/1993
• Est. Priority Date: 05/15/1990
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Fritz, Michael, Mosoiu, Dan, Vogel, Peter, Thym, Detlef
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): LE, LONG V
• Application Number:
  US07/698,466
• Time in Patent Office: 739 Days
• Field of Search: 422/56, 422/55, 422/57, 422/58, 436/169, 436/72, 436/170, 435/805, 428/327, 552/302, 552/295
• US Class Current: 422/426
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

The invention concerns a test carrier for the determination of ions containing a test layer which has a liquid-resistant, organic phase which contains a hydrophobic polymer in a homogeneous mixture with a hydrophobic, organic liquid of low volatility and an ionophore, as well as a substance which changes its colour in the presence of the ion to be determined. The test carrier is characterized in that the test layer contains particles with an oil number of 80-200 and the ionophore is homogeneously dispersed in the hydrophobic, organic phase.

Claims

1. A test carrier useful in determining a specific ion in a liquid sample comprising:

a porous film layer having a liquid resistant organic phase which contains a homogeneous mixture of

(i) a hydrophobic polymer,

(ii) a plasticizer,

(iii) an ionophore,

(iv) a substance which changes color in the presence of the ion to be determined, and

(v) a plurality of particles having an oil absorption value from 80-200, wherein said ionophore is homogeneously dispersed in said organic phase.

2. Test carrier of claim 1, wherein said hydrophobic polymer is a copolymer of vinyl acetate.

3. The test carrier of claim 1 comprising porous particles.

4. Test carrier of claim 1, wherein said hydrophobic polymer and said plurality of particles are present in a weight ratio ranging from about 5:

1 to about 1;

10.

5. Test carrier of claim 4, wherein said weight ratio ranges from about 1:

1 to about 1;

3.

6. Test carrier of claim 1, wherein said plasticizer is selected from the group consisting of a setacic acid ester, a phthalic acid ester, an acrylic acid ester, a phosphoric acid ester and a silicon.

7. Test carrier of claim 1, wherein said hydrophobic polymer and said plasticizer are present in a weight ratio ranging from about 5:

1 to about 1;

5.

8. Test carrier of claim 7, wherein said weight ratio is from about 2:

1 to about 1;

2.

9. Test carrier of claim 1, wherein said substance which changes color is of the formula ##STR7## wherein R¹, R² and R³ are the same or different and are selected from the group consisting of hydrogen, an alkyl group and a alkoxy group, wherein at least one of R¹, R² and R³ is an alkyl or alkoxy group having from 8 to 30 carbons,

R⁴ is hydrogen or an alkyl group,

R⁵ is a nitro group, an alkyl group substituted by halogen, a cyano group, a sulfonamide group or an alkyl sulfonyl group,

X is nitrogen or CR⁶, and

Y is sulphur or CR⁷ =CR⁸

wherein R⁶, R⁷ and R⁸ are the same or different and are selected from the group consisting of hydrogen, halogen, a nitro group, a cyano group, an unsbustituted alkyl group, an alkyl group substituted by halogen and an alkyl sulfonyl group.

10. The test carrier of claim 1 wherein said test layer further comprises an alkyl phenol having at least two nitro groups, an alkoxynaphthol having at least two nitro groups or a carbonyldicyanidophenyl hydrazone.

11. The test carrier of claim 1 wherein said test layer further comprises a buffer capable of maintaining the pH at a value of between 5-10.

13. Method for determining a specific ion in a liquid sample comprising contacting said liquid sample to the test carrier of claim 1 and determining a change in color of said substance which changes color in the presence of said specific ion.

15. Method of claim 13, comprising determining said change in color about 60 seconds after contacting the liquid sample thereto.

17. Test carrier of claim 1 wherein the plasticizer is 2,2-diphenyl-1-cyano-acrylic acid-ethylhexylester.

12. A test carrier useful in determining a specific ion in a liquid sample comprising:

(a) a first test layer which is a porous film layer having a liquid resistant organic phase which contains homogenous mixture of;

(i) a hydrophobic polymer,

(ii) a plasticizer,

(iii) an ionophore, and

(iv) a plurality of particles having an oil absorption value of from 80-200, wherein said ionophore is homogeneously dispersed in said organic phase, and

(b) a second test layer containing a substance which changes color in the presence of said specific ion, wherein said first and second layers are positioned in said test carrier to permit fluid contact therebetween.

14. Method for determining a specific ion in a liquid sample comprising contacting said liquid sample to the test carrier of claim 12 and determining a change in color of said substance which changes color in the presence of said specific ion.

16. Method of claim 14, comprising determining said change in color about 60 seconds after contacting the liquid sample thereto.