Automatic chemical analyzer having an improved delivery mechanism

• US 5,213,761 A
• Filed: 04/09/1990
• Issued: 05/25/1993
• Est. Priority Date: 04/12/1989
• Status: Expired due to Term

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Olympus Optical Corporation Limited (Olympus Corporation)
• Inventors: Sakagami, Toshio
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): REDDING, DAVID A
• Application Number:
  US07/506,077
• Time in Patent Office: 1,142 Days
• Field of Search: 422/64, 422/100, 422/103, 422/63, 422/67, 73/864.14, 73/864.11, 73/864.24, 436/54, 436/49, 436/47
• US Class Current: 422/63
• CPC Class Codes: G01N 2035/0441 for samples
• CPC Class Codes: G01N 2035/0443 for reagents
• CPC Class Codes: G01N 2035/0444 for cuvettes or reaction vessels
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

An automatic chemical analyzer including a reaction turntable having a plurality of reaction vessels arranged along a periphery thereof, a sample turntable having a plurality of sample vessels arranged along the periphery thereof, a reagent turntable having a plurality of reagent bottles in which different kinds of reagents are contained, a sample delivery mechanism for delivering a given amount of a sample into a reaction vessel, and a reagent delivery mechanism for delivering a given amount of a reagent into the sample to form a test liquid in the reaction vessel. The reagent delivery mechanism has a nozzle which can be moved between a reagent sucking position above a reagent bottle and a reagent discharging position above a reaction vessel; a plurality of detachable probes which are arranged in respective reagent bottles in such a manner that the tip of the nozzle can be inserted into a probe in a reagent bottle at the reagent sucking position; a syringe connected to the nozzle for sucking a given amount of a reagent into the probe coupled with the nozzle; and a probe removing mechanism for removing the probe from the tip of the nozzle after the reagent has been discharged into the reaction vessel and the probe has been returned into the reagent bottle from which the probe was picked-up by the nozzle.

Claims

1. In an automatic chemical analyzer including a plurality of sample vessels, a plurality of reaction vessels, and a plurality of reagent bottles, a sample holding means for holding said plurality of sample vessels in which a plurality of samples to be analyzed are contained, a reaction vessel holding means for holding said plurality of reaction vessels along a reaction line, a sample delivery means for delivering the samples held in the sample vessels in the sample holding means into the reaction vessels on the reaction line, a reagent holding means for holding said plurality of reagent bottles which contain a plurality of different kinds of reagents, a reagent delivery means for delivering the reagents into the reaction vessels on the reaction line, and a measuring means for measuring substances contained in a reaction liquid which is a mixture of a sample and at least one reagent and is contained in a reaction vessel, the improvement wherein said delivery means comprises

a delivery nozzle;

a pump means connected to said delivery nozzle for sucking and discharging reagents;

a plurality of detachable probes which are constructed to be detachably coupled with a tip of said delivery nozzle;

a plurality of probe holding members each of which is contained in one of said reagent bottles and holds one of said detachable probes when said probe is not in use, said probes being returned to said probe holding members after use;

a delivery nozzle moving means for moving the delivery nozzle between two positions, a first position at which the tip of the delivery nozzle can be coupled with a probe held in a probe holding member contained in a reaction bottle which contains a reagent to be delivered into a reagent vessel and a given amount of the reagent is sucked into the probe by operating said pump means, and a second position at which the probe is situated above the reaction vessel and the sucked reagent is discharged into the reaction vessel by operating said pump means; and

a probe removing means for removing the probe from the tip of the delivery nozzle into the probe holding member from which the probe has been removed;

wherein the detachable probe has a tube-like main body having upper and lower openings and a flange formed at an upper edge of the main body, and

said probe holding member has a tube-like main body having upper and lower openings and a flange formed at an upper end of the main body, the flange of the probe being placed on the flange of the probe holding member, the length of the probe holding member being such that a tip of the tube-like main body is situated slightly above the bottom wall of the container,

said probe and probe holding member being so constructed that a lower end of the probe protrudes from the lower opening of the probe holding member when the probe is set in the probe holding member.

2. An analyzer according to claim 1, wherein said delivery nozzle moving means comprises a first driving means for rotating the delivery nozzle in a horizontal plane between said first and second positions and second driving means for moving the delivery nozzle up and down at said first position.

3. An analyzer according to claim 2, wherein said detachable probe has an upper mouth which is widened upward and the tip of delivery nozzle is thinned downward so that the tip of delivery nozzle can be easily and firmly inserted into the upper mouth of the probe when the delivery nozzle is moved downward by said second driving means.

4. An analyzer according to claim 1, wherein each of said reagent bottles has an inclined inner bottom surface and a recess formed in the inclined inner bottom surface at such position that the lower end of the probe is slightly projected into the recess.

5. An analyzer according to claim 1, wherein said probe removing means comprises a fork plate having a fork having a gap which is larger than the diameter of said tube-like main body of the probe, but is smaller than the diameter of the flange of the probe, and a solenoid for moving the fork plate with respect to a locus of the up and down movement of the probe.

6. An analyzer according to claim 1, wherein said reagent holding means is a circular member, said reagent bottles being arranged side by side along a sector of the circular member along which said nozzle is moved.

7. An analyzer according to claim 1, wherein said reagent holding means is a turntable, said reagent bottles being arranged on said turntable, said turntable being rotated to index any one of said reagent bottles into said first position.

8. In an automatic chemical analyzer including a plurality of sample vessels, a plurality of reaction vessels, and a plurality of reagent bottles, a sample holding means for holding said plurality of sample vessels in which a plurality of samples to be analyzed are contained, a reaction vessel holding means for holding said plurality of reaction vessels along a reaction line, a sample delivery means for delivering the samples held in the sample vessels in the sample holding means into the reaction vessels on the reaction line, a reagent holding means for holding said plurality of reagent bottles which contain a plurality of different kinds of reagents, a reagent delivery means for delivering the reagents into the reaction vessels on the reaction line, and a measuring means for measuring substances contained in a reaction liquid which is a mixture of a sample and at least one reagent and is contained in a reaction vessel, the improvement wherein said delivery means comprise

a delivery nozzle;

pump means connected to said delivery nozzle for sucking samples and reagents;

a plurality of detachable sample delivery probes which are constructed to be detachably coupled with a tip of said delivery nozzle;

a plurality of detachable reagent delivery probes which are constructed to be detachably coupled with the tip of said delivery nozzle;

a plurality of sample delivery probe holding members each of which is contained in one of said sample vessels and holds one of said detachable sample delivery probes when said sample delivery probe is not in use, said sample delivery probe being returned to said sample delivery probe holding member after use;

a plurality of reagent delivery probe holding members each of which is contained in one of said reagent bottles and holds one of said detachable reagent delivery probes when said reagent delivery probe is not in use, said reagent delivery probe being returned to said reagent delivery probe holding member after use;

a delivery nozzle moving means for moving the delivery nozzle among four positions, a first position at which the tip of the delivery nozzle can be coupled with a sample delivery probe held in a sample delivery probe holding member contained in a sample vessel which contains a sample to be delivered into a reaction vessel, a second position at which the sample delivery probe is immersed into the sample contained in said sample vessel and a given amount of the sample is sucked into the sample delivery probe by operating said pump means, a third position at which the tip of the delivery nozzle can be coupled with a reagent nozzle delivery probe held in a reagent delivery probe holding member contained in a reagent bottle which contains a reagent to be delivered into the reaction vessel and a given amount of the reagent is sucked into the reagent delivery probe by operating said pump means, and a fourth position at which the sample delivery probe and the reagent delivery probe are situated above the reaction vessel and the sucked sample and reagent are discharged into the reaction vessel;

a sample delivery probe removing means for removing the sample delivery probe from the tip of the delivery nozzle into the sample delivery probe holding member from which the sample delivery probe has been removed; and

a reagent delivery probe removing means for removing the reagent delivery probe from the tip of the delivery nozzle into the reagent delivery probe holding member from which the reagent probe has been removed;

wherein the reagent delivery probe has a tube-like main body having upper and lower openings and a flange formed at an upper edge of the main body, and

said reagent delivery probe holding member has a tube-like main body having upper and lower openings and a flange formed at an upper end of the main body, the flange of the reagent delivery probe being placed on the flange of the reagent delivery probe holding member, the length of the delivery probe holding member being such that a tip of the tube-like main body is situated slightly above the bottom wall of the container, `said reagent delivery probe and said reagent delivery probe holding member being so constructed that the lower end of the reagent delivery probe protrudes from the lower opening of the reagent delivery probe holding member when the reagent delivery probe is set in the reagent delivery probe holding member.

9. An analyzer according to claim 8, wherein said delivery nozzle moving means comprises a first driving means for rotating the delivery nozzle in a horizontal plane among said first to fourth positions and second driving means for moving the delivery nozzle up and down at said first, second and third positions.

10. An analyzer according to claim 9, wherein each of said detachable reagent delivery probes has an upper mouth which is widened upward and the tip of the delivery nozzle is thinned downward so that the tip of the delivery nozzle can be easily and firmly inserted into the upper mouth of the reagent delivery probe when the delivery nozzle is moved downward by said second driving means.

11. An analyzer according to claim 8, wherein each of said reagent bottles has an inclined inner bottom surface and a recess formed in the inclined inner bottom surface at such position that the lower end of the reagent delivery probe is slightly projected into the recess.

12. An analyzer according to claim 8, wherein said reagent delivery probe removing means comprises a fork plate having a gap which is larger than the diameter of the tube-like main body of the reagent delivery probe, but is smaller than the diameter of the flange of the reagent delivery probe, and a solenoid for moving the fork plate with respect to a locus of the up and down movement of the reagent delivery probe.

13. An analyzer according to claim 8, wherein said reagent holding means is a turntable, said reagent bottles being arranged on said turntable, said turntable being rotated to index any one of said reagent bottles into said third position.