Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays

• US 5,215,882 A
• Filed: 11/30/1989
• Issued: 06/01/1993
• Est. Priority Date: 11/30/1989
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Original Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Lang, Rhonda, Bahl, Chander, Mendoza, Leopoldo G.
• Primary Examiner(s): YARBROUGH, AMELIA
• Application Number:
  US07/444,031
• Time in Patent Office: 1,279 Days
• Field of Search: 435/6, 435/174, 435/810, 935/77, 935/78, 935/108, 436/178, 436/518, 436/530, 436/808, 424/78, 424/82
• US Class Current: 435/6.16
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.

Claims

1. A method of immobilizing nucleic acid to a solid surface having free aldehyde groups comprising the steps of:

(a) providing a modified nucleic acid strand comprising a variable portion and an anchor portion wherein said variable portion comprises a nucleotide sequence having a selected base sequence and said anchor portion comprises from two to about 10 nucleotide bases modified with a primary amine function or nucleotide base equivalents having a primary amine function; and

(b) reacting the primary amine functions of said anchor portion of said modified nucleic acid strand with the free aldehyde groups of said solid surface in a preselected pattern in the presence of a reducing agent to form complexes of said modified nucleic acid strand and at least a portion of said free aldehyde groups, such that said modified nucleic acid strand is attached to said solid surface through the primary strand functions of said anchor portion.

2. The method of claim 1 wherein said reducing agent is sodium cyanoborohydride.

3. The method of claim 1 wherein said solid surface is cellulose paper having free aldehyde groups on its surfaces.

4. The method of claim 1 wherein said anchor portion comprises from about five to about eight nucleotide bases or nucleotide base equivalents.

5. The method of claim 1 wherein said nucleotide base or nucleotide base equivalent is cytosine having nitrogen at the exocyclic 4 position of the pyrimidine ring modified with a substituent having a primary amine function.

6. The method of claim 1 wherein said variable portion is from about two to about 1,000 nucleotide bases in length.

7. The method of claim 6 wherein said variable portion is from about 15 to about 100 nucleotide bases in length.

8. The method of claim 1 wherein said variable portion of the modified nucleotide strand is a homopolymer.

9. The method of claim 8 wherein said homopolymer is poly thymidine.

10. The method of claim 8 wherein said homopolymer is poly adenine.

11. The method of claim 1 wherein said variable portion is RNA.

12. The method of claim 1 wherein said variable portion is DNA.

13. The method of claim 1 wherein said variable portion is of human origin.

14. The method of claim 1 wherein said variable portion is of bacterial origin.

15. The method of claim 1 wherein said variable portion is of viral origin.

16. The method of claim 1 wherein said variable portion is of fungal origin.

17. The method of claim 1 further comprising the step of reacting unreacted aldehyde groups from step (b) with a mixture of an amino acid and a reducing agent.

18. The method of claim 17 wherein said amino acid is α

-amino caproic acid.

19. A solid surface for conducting nucleic acid hybridization assays comprising

a solid surface prepared in accordance with the method of claim 1.

20. A solid surface for conducting nucleic acid hybridization assays comprising

a solid surface prepared in accordance with the method of claim 8.

21. A kit for performing a nucleic hybridization assay comprising,

a solid surface of claim 19 and at least one labeled nucleic acid probe, said solid surface and said at least one nucleic acid probe selected to form a detection system for a target strand of nucleic acid.

22. A kit for performing a nucleic hybridization assay comprising,

a solid surface of claim 20 and at least one labeled nucleic acid probe, said solid surface and said at least one nucleic acid probe selected to form a detection system for a target strand of nucleic acid.