Catalytic and reactive polypeptides and methods for their preparation and use
First Claim
1. A polypeptide capable of promoting a chemical reaction involving the conversion of one or more reactants to one or more products, said polypeptide having a binding site specific for at last one reactant or reaction intermediate, wherein the polypeptide is other than a naturally occurring enzyme and includes at least one active functionality covalently attached to the polypeptide proximate the binding site, which functionality is capable of chemically participating in the chemical reaction or catalytically enhancing the chemical reaction rate, wherein the functionality is either a moiety covalently attached to an amino acid side chain proximate the binding site or an amino acid side chain other than a naturally occurring amino acid side chain and wherein the polypeptide is an antibody or antibody fragment.
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Abstract
Catalytic and reactive polypeptides include a binding site specific for a reactant or reactive intermediate involved in a chemical reaction of interest. The polypeptides further include at least one active functionality proximate the bi
This invention was made with Government support under Grant Contract No. AI-24695 awarded by the Department of Health and Human Services, under Grant Contract No. N 00014-87-K-0256, awarded by the Office of Naval Research. The Government has certain rights in this invention, under Grant Contract CHE 8822412 awarded by the National Science Foundation, and under Grant Subcontract C87-101226 awarded by the Department of Energy.
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Citations
19 Claims
- 1. A polypeptide capable of promoting a chemical reaction involving the conversion of one or more reactants to one or more products, said polypeptide having a binding site specific for at last one reactant or reaction intermediate, wherein the polypeptide is other than a naturally occurring enzyme and includes at least one active functionality covalently attached to the polypeptide proximate the binding site, which functionality is capable of chemically participating in the chemical reaction or catalytically enhancing the chemical reaction rate, wherein the functionality is either a moiety covalently attached to an amino acid side chain proximate the binding site or an amino acid side chain other than a naturally occurring amino acid side chain and wherein the polypeptide is an antibody or antibody fragment.
- 5. A polypeptide having a binding site and an active functionality covalently attached to a side chain of an amino acid proximate the binding site, wherein the binding site is capable of complexing a reactant or reaction intermediate in a chemical reaction and the active functionality is capable of enhancing the rate of a chemical reaction, and wherein the polypeptide is an antibody or an antibody fragment.
- 12. A method for enhancing the rate of a chemical reaction involving the conversion of one or more reactants to one or more products, said method comprising exposing a reaction mixture including said reactant(s) to a polypeptide selected from the group consisting of antibodies and antibody fragments and having a binding site capable of forming a complex with said reactant(s) or a reaction intermediate including said reactant(s), wherein an active functionality is a side chain of a non-natural amino acid proximate the binding site or is a moiety covalently attached to a side chain of an amino acid proximate the binding site, said active functionality being capable of promoting the conversion of reactant(s) to product(s).
Specification