Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
First Claim
Patent Images
1. A method for using nucleic acid encoding an inhibin β
- A chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-70, 70-110, 110-180, 180-260, 270-309, 28-58, 61-87, 90-108, 111-179, 182-213, 216-258, and 28-87 of FIG. 2B, and amino acid residues 1-30, 32-40, 43-59, 62-80, 83-185, and 186-230 of FIG. 8 to produce the prodomain polypeptide comprising (a) constructing a vector that comprises said nucleic acid, (b) transforming a host cell with the vector, and (c) culturing the transformed cell under conditions appropriate for expression of said polypeptide.
0 Assignments
0 Petitions
Accused Products
Abstract
DNA encoding the prepro inhibin α and β chains has been isolated. This DNA is ligated into expression vectors and used to transform host cells for the preparation of inhibin or activin. Also provided are prohormone domains and other inhibin α and β chain derivatives having therapeutic or diagnostic interest. The compositions provided herein are useful in the manipulation of fertility in animals.
-
Citations
17 Claims
-
1. A method for using nucleic acid encoding an inhibin β
- A chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-70, 70-110, 110-180, 180-260, 270-309, 28-58, 61-87, 90-108, 111-179, 182-213, 216-258, and 28-87 of FIG. 2B, and amino acid residues 1-30, 32-40, 43-59, 62-80, 83-185, and 186-230 of FIG. 8 to produce the prodomain polypeptide comprising (a) constructing a vector that comprises said nucleic acid, (b) transforming a host cell with the vector, and (c) culturing the transformed cell under conditions appropriate for expression of said polypeptide.
- View Dependent Claims (2, 3, 4, 5, 6, 7)
-
8. Non-chromosomal DNA encoding an inhibin β
- A chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-70, 70-110, 110-180, 180-260, 270-309, 28-58, 61-87, 90-108, 111-179, 182-213, 216-258, and 28-87 FIG. 2B, and amino acid residues 1-30, 32-40, 43-59, 62-80, 83-185, and 186-230 of FIG. 8.
- View Dependent Claims (9, 10, 11)
-
12. A replicable vector comprising DNA encoding an inhibin β
- A chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-70, 70-110, 110-180, 180-260, 270-309, 28-58, 61-87, 90-108, 111-179, 182-213, 216-258, and 28-87 of FIG. 2B, and amino acid residues 1-30, 32-40, 43-59, 62-80, 83-185, and 186-230 of FIG. 8.
- View Dependent Claims (13, 14)
-
15. A host cell transformed with a replicable vector comprising DNA encoding an inhibin β
- A chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-70, 70-110, 110-180, 180-260, 270-309, 28-58, 61-87, 90-108, 111-179, 182-213, 216-258, and 28-87 of FIG. 2B, and amino acid residues 1-30, 32-40, 43-59, 62-80, 83-185, and 186-230 of FIG. 8.
- View Dependent Claims (16, 17)
Specification