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Nucleic acid amplification employing ligatable hairpin probe and transcription

  • US 5,215,899 A
  • Filed: 08/23/1990
  • Issued: 06/01/1993
  • Est. Priority Date: 11/09/1989
  • Status: Expired due to Fees
First Claim
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1. A nucleic acid probe comprising (1) a single stranded self-complementary sequence capable of forming, under hybridizing conditions, a hairpin structure having a functional promoter region, and (2) a single stranded probe sequence extending from the 3'"'"' end of said self-complementary sequence, whereby upon hybridization of a target sequence complementary to said probe sequence and ligation of the resulting hybridized target sequence to the 5'"'"' end of the hairpin-forming self-complementary sequence, said target sequence is transcribable in the presence of an RNA polymerase and required ribonucleoside triphosphates under said hairpin-forming hybridizing conditions.

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