Convenient determination of trace lead in whole blood and other fluids
First Claim
1. A surface-modified bioelectrode for detecting nanomolar levels of lead or mercury ion consisting essentially of a colloidal gold adsorbed enzyme deposited onto an d in contact with a conducting surface to form a coating thickness of between about 4-12 monolayer a on said conducting surface wherein inhibition of the enzyme detectably affects current generated from redox reactions catalyzed by the enzyme when the surface modified bioelectrode is suitably coupled wit a reference electrode.
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Abstract
The invention relates to novel b of detecting metal ion concentrations less than about 10 μg/dl fluid. Biosensors based on the enzyme isocitrate dehydrogenase are particularly suited for detecting trace lead ion concentrations in water and in blood. Bioelectrodes are fabricated from surface deposited colloidal gold adsorbed enzyme that retains high catalytic activity Other aspects of the invention include detection devices for convenient and rapid measurement of metal ions.
52 Citations
30 Claims
- 1. A surface-modified bioelectrode for detecting nanomolar levels of lead or mercury ion consisting essentially of a colloidal gold adsorbed enzyme deposited onto an d in contact with a conducting surface to form a coating thickness of between about 4-12 monolayer a on said conducting surface wherein inhibition of the enzyme detectably affects current generated from redox reactions catalyzed by the enzyme when the surface modified bioelectrode is suitably coupled wit a reference electrode.
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14. A bioelectrode of the detection of lead ion concentrations of a least 10 μ
- g/dl consisting essentially of colloidal gold adsorbed isocitrate dehydrogenase deposited to form a coating thickness of between about 4-12 nonlayers on a glassy carbon surface with AND or NADP.
- View Dependent Claims (15, 16, 17)
Specification