Cell stretching apparatus

• US 5,217,899 A
• Filed: 08/24/1990
• Issued: 06/08/1993
• Est. Priority Date: 08/24/1990
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology, Massachusetts General Hospital (Mass General Brigham, Inc.), The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: Massachusetts Institute of Technology, The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Venegas, Jose G., Wright, John D., Shapiro, Alan R., Melendez, Luis A., Gray, Martha L., Schaffer, Jonathan L.
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): BEISNER, WILLIAM H
• Application Number:
  US07/572,683
• Time in Patent Office: 1,019 Days
• Field of Search: 435/284, 435/285, 435/287, 435/299, 435/296-298, 435/300, 435/301, 435/310, 435/316
• US Class Current: 435/283.1
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Abstract

Disclosed is an apparatus and method for imparting to a living culture of cells biaxial mechanical forces which approximate the mechanical forces to which cells are subjected in vivo. The apparatus includes a displacement applicator which may be actuated to contact and stretch a membrane having a living cell culture mounted thereon. Stretching of the membrane imparts biaxial mechanical forces to the cells. These forces may be uniformly applied to the cells, or they may be selectively non-uniformly applied.

Claims

1. An apparatus for applying cyclical mechanical stretching forces to living cells, comprising

at lest one elastic, biocompatible membrane capable of receiving a culture of living cells and culture medium for said cells, said membrane being suitable for cells to adhere to and grow upon said membrane;

clamp means for securing the membrane about its entire periphery within the apparatus;

at least one selectively displaceable applicator means, constructed of a low friction material, for applying a stretching force to said membrane such that the applicator means is a rigid member that applies biaxial strain of the cells growing upon the surface of the membrane, in a uniform or selectively non-uniform profile, said applicator means having a membrane-facing surface with a size slightly less than a shape substantially corresponding to an area of the membrane exposed by the clamp means; and

force generating means for cyclically moving said applicator means to contact and control displacement of said membrane.

2. The apparatus of claim 1 wherein the membrane-facing surface of the applicator means and the portion of the membrane contacted by the applicator means are circular in shape.

3. The apparatus of claim 2 wherein the membrane-facing surface of said applicator means is irregular and has one or more elevated ridges which contact said membrane.

4. The apparatus of claim 3 wherein at least one raised, membrane contacting ridge is disposed about the perimeter of the membrane-facing surface of the applicator means.

5. The apparatus of claim 4 wherein said ridge is elevated about 0.125 inch above the surface of said applicator means.

6. The apparatus of claim 1 wherein said applicator means further includes a downwardly projecting member attached to a bottom surface of said applicator means.

7. The apparatus of claim 6 wherein said force generating means includes a cam means for contacting said downwardly projecting member to enable the member and the applicator means to move upwardly and downwardly.

8. The apparatus of claim 1 further including a lubricating material applied to the membrane facing surface of said applicator means.

9. The apparatus of claim 1 wherein the clamp means for securing the membrane includes a removable and disposable ring-shaped structure, secured to said apparatus, and having said membrane adhered thereto.