Sensor element and method for making the same

• US 5,219,527 A
• Filed: 08/28/1991
• Issued: 06/15/1993
• Est. Priority Date: 08/16/1989
• Status: Expired due to Fees

• Current Assignee: Pierce Biotechnology Incorporated (Perspecta, Inc.)
• Original Assignee: Puritan Bennett Corp. (Medtronic Plc)
• Inventors: Bankert, Charles S., Hui, Henry K.
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/758,291
• Time in Patent Office: 657 Days
• Field of Search: 422/58, 422/82.05, 422/82.07, 422/82.08, 422/82.06, 422/57, 385/13, 427/2, 436/68, 436/165, 436/166, 128/634
• US Class Current: 422/82.06
• CPC Class Codes: G01N 2021/174 either absorption-reflection or emission-fluorescence
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 2021/775 Indicator and selective membrane
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

A sensor element includes an analyte-permeable matrix which supports and maintains a sensing solution containing an analyte-sensitive indicator substance in a plurality of tiny vesicles. The sensor element is prepared by combining the sensing solution with an emuslifier that renders the solution dispersed within the matrix material. The sensing solution remains suspended in these dispersed vesicles after the matrix solidifies. A sensor element made in accordance with the present invention may be placed at the end of an optical fiber.

Claims

1. A sensor element comprising:

an optical fiber having a distal portion coated with a matrix made from a hydrophobic material that is permeable to at least one analyte;

a first plurality of vesicles disposed in said matrix containing a first aqueous sensing solution with a first fluorescent dye indicator that is responsive to a particular analyte; and

a second plurality of vesicles disposed in said matrix containing a second aqueous sensing solution with a second fluorescent dye indicator that is responsive to a second analyte, said vesicles being dispersed throughout said matrix, said matrix being only slightly thicker than the size of one of said vesicles.

2. A sensor element as defined in claim 1 wherein each said sensing solution and the matrix material are mutually immiscible.

3. A sensor element as defined in claim 1 wherein each of said first and second sensing solutions contains an emulsifier that forms an emulsion of said first and second sensing solutions with said matrix, and that renders each of said sensory solutions immiscible with said matrix and stabilizes the resulting emulsion.

4. A sensor element as defined in claim 3 wherein the emulsifier is a water soluble or water swellable polymer.

5. A sensor element as defined in claim 3 wherein the emulsifier is selected from a group consisting of polyvinylpyrrolidone, polytheylene glycol and polyethylene oxide.

6. A method for making a sensor element comprising the steps of:

combining a first sensing solution including an indicator substance that is responsive to an analyte with an emulsifier solution to form a first emulsified solution;

mixing a liquified matrix that is immiscible with the first sensing solution, and that is permeable to at least one analyte with the first emulsified solution;

combining a second sensing solution that is immiscible with the liquified matrix and the first sensing solution, and having an indicator that is receptive to a second analyte with an emulsifier to form a second emulsified solution;

mixing the second emulsified solution with the liquified matrix and the first emulsified solution;

dispersing the first and second emulsified solutions as a plurality of vesicles in the liquified matrix material; and

causing the liquified matrix to solidify in a thickness only slightly greater than the size of one of said vesicles.

7. The method of claim 6 wherein the first and second emulsified solutions are homogeneously dispersed throughout the matrix.

8. The method of claim 6 further comprising, before the dispersing step, the step of:

mixing a catalyst to the emulsified solutions and liquified matrix.