×

Physical mapping of complex genomes

  • US 5,219,726 A
  • Filed: 06/02/1989
  • Issued: 06/15/1993
  • Est. Priority Date: 06/02/1989
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for simultaneous identification of overlapping cosmid clones among multiple cosmid clones, comprising:

  • (a) arranging the multiple cosmid clones, whereby each clone may be identified and replicas of said arrangement may be generated;

    (b) pooling a first portion of the multiple cosmid clones and synthesizing mixed end-specific RNA probes from the DNA inserts that have been prepared from said pooled clones, wherein said portion includes less than all of said multiple cosmid clones;

    (c) hybridizing the probes to a replica of said arranged cosmid clones and identifying the cosmid clones in the replica that hybridize to the probes, wherein said identified clones include the pooled cosmid clones and cosmid clones that contain DNA inserts that overlap with the DNA inserts in the pooled clones;

    (d) repeating said hybridization step with a second portion of mixed end-specific probes that are prepared from a second pooled portion of multiple cosmid clones; and

    (e) identifying the cosmid clones in each replica to which both the probes of steps b) and d) hybridize thereby identifying overlapping clones.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×