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Agglutination method for the determination of multiple ligands

  • US 5,219,763 A
  • Filed: 08/03/1990
  • Issued: 06/15/1993
  • Est. Priority Date: 11/04/1988
  • Status: Expired due to Fees
First Claim
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1. A method for determining the presence or absence of each of a predetermined number of three or more ligands in a liquid, semi-liquid or pasty specimen, by a direct or indirect aggultination, or an aggultination inhibition method by competition, comprising the steps:

  • contacting the specimen with a predetermined number of homogenous populations of fluorescent beads having a specific fluorescence and one or more pre-determined antiligands fixed to their surface, said predetermined number of populations being less than said predetermined number of ligands and wherein at least one of said ligands is capable of binding to the antiligand on two or more of said homogeneous populations of beads;

    analyzing said specimen containing said beads using a measuring means capable of discriminating between the specific fluorescence of said beads for each ligand;

    determining in response to said analyzing step the number of non-agglutinated beads, the number of agglutinated beads, the number of bead aggregates and for each aggregate, the number of the beads it comprises; and

    correlating the information determined in said determining step with the presence or absence in said specimen, of each of said different ligands to thereby enable the simultaneous determination of a plurality of ligands using a single bead contacting step.

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