Electrophoretic media

• US 5,219,923 A
• Filed: 08/15/1990
• Issued: 06/15/1993
• Est. Priority Date: 04/29/1988
• Status: Expired due to Term

• Current Assignee: Cambrex Bio Science Rockland, Inc.
• Original Assignee: At Biochem Co.
• Inventors: Shorr, Robert
• Primary Examiner(s): Bleutge, John C.
• Assistant Examiner(s): SWEET, MARK D
• Application Number:
  US07/568,237
• Time in Patent Office: 1,035 Days
• Field of Search: 524/458, 524/827, 524/831
• US Class Current: 524/827
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10S 524/916 Hydrogel compositions

Abstract

Electrophoretic media based on polymers with novel structures are disclosed. The polymers are formed by polymerization of acrylamide monomers, such as N-methylolacrylamide, with cross-linking agents or comonomer agents such as tetraethylene diacrylate and bisacrylamide methylether.

Claims

1. An electrophoretic medium consisting essentially of an aqueous gel formed by polymerization in the presence of aqueous medium and in the absence of oxygen of one or more acrylamide monomers in the presence of one or more different crosslinking or comonomer agents selected from the group consisting of diethyleneglycol diacrylate, diacetone acrylamide, pentaerythritolacrylate, 1,3-butyleneglycol diacrylate, tetraethylene glycol diacrylate, bisacrylamide methylether and tris-(2-hydroxyethyl)isocyanurate triacrylate.

2. An electrophoretic medium of claim 1 wherein said acrylamide monomer is N-methylolacrylamide.

3. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises diethyleneglycol diacrylate.

4. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises diacetone acrylamide.

5. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises pentaerythritolacrylate.

6. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises 1,3-butyleneglycol diacrylate.

7. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises tetraethylene glycol diacrylate.

8. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises tris-(2-hydroxyethyl)isocyanurate triacrylate.

9. An electrophoretic medium of claim 1 wherein said crosslinking agent or comonomer comprises bisacrylamide methylether.

10. An electrophoretic medium of claim 7 wherein said acrylamide monomer is N-methylolacrylamide.

11. An electrophoretic medium of claim 9 wherein said acrylamide monomer is N-methylolacrylamide..

12. A polymerization mixture for preparing the electrophoretic medium of claim 1 comprising one or more acrylamide monomers and one or more crosslinking agents selected from the group consisting of diethyleneglycol diacrylate, diacetone acrylamide, pentaerythritolacrylate, 1,3-butyleneglycol diacrylate, tetraethylene glycol diacrylate and tris-(2-hydroxyethyl)isocyanurate triacrylate;

a polymerization catalyst; and

aqueous medium.