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Differential separation assay

  • US 5,221,454 A
  • Filed: 06/25/1992
  • Issued: 06/22/1993
  • Est. Priority Date: 01/31/1992
  • Status: Expired due to Term
First Claim
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1. A diagnostic system for determining presence of an analyte comprising,an excess amount of fluorescence labeled binding agent forming a complex with a specific target analyte to be determined, the combination having free fluorescence labeled binding agent and bound analyte,an elongated migration path for the bound analyte and the labeled binding agent, the bound analyte and the labeled binding agent having different migration rates, said path having a migration start zone and a downstream migration analysis zone,means for effecting the migration of the bound analyte and the free labeled binding agent,optical means in communication with the analysis zone for timing the migration of fluorescence labeled binding agent and bound analyte from the start zone to the analysis zone, andmeans for comparing the migration times or rates of fluorescence labeled binding agent and bound analyte, whereby the presence of analyte is determined.

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