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Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms

  • US 5,221,623 A
  • Filed: 07/19/1989
  • Issued: 06/22/1993
  • Est. Priority Date: 07/22/1986
  • Status: Expired due to Fees
First Claim
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1. An aerobic or facultative anaerobic microoganism which is capable of homologous recombination, transformed by the integration of an isolated foreign DNA fragment, within any of its chromosomal regions, wherein integration of transforming DNA does not alter cell viability, said foreign DNA fragment comprising lux AB genes of any vibrio bioluminescent bacterium fused in operable linkage with an active promoter, and further comprising an antibiotic resistance marker, wherein said active promoter is selected from the group consisting of constitutive promoters of gene expression and inducible promoters of gene expression, and is active in the transformant.

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