Capillary mixing device

• US 5,222,808 A
• Filed: 04/10/1992
• Issued: 06/29/1993
• Est. Priority Date: 04/10/1992
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Biotrack, Inc. (Novartis Ag)
• Inventors: Sugarman, Jeffrey, Gibbons, Ian
• Primary Examiner(s): JENKINS, ROBERT
• Application Number:
  US07/867,155
• Time in Patent Office: 445 Days
• Field of Search: 366/273, 366/274, 422/99, 422/100, 422/102, 422/101, 435/287, 435/315, 435/316
• US Class Current: 366/274
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/451 wherein the mixture is directly exposed to an electromagnetic field without use of a stirrer, e.g. for material comprising ferromagnetic particles or for molten metal
• CPC Class Codes: B01F 35/7172 using capillary forces

Abstract

A capillary mixing device, comprising a liquid impervious housing; an interior space in the housing comprising a chamber in the housing having capillary spacing in one dimension and non-capillary spacing in other dimensions; and a plurality of magnetic or magnetically inducible particles in the chamber. The chamber is normally accessed through one or more capillary passageways leading to a surface of the housing and is adapted to be retained by a magnetic device that comprises means for generating a moving magnetic field and means for retaining the chamber device in an orientation so that the magnetic field has a field vector that intersects the capillary chamber perpendicular to the dimension having capillary spacing.

Claims

1. A system capable of carrying out mixing in a capillary chamber, comprising:

a. a chamber device, comprising;

i. a liquid impervious housing;

ii. a chamber in said housing having capillary spacing in one dimension and non-capillary spacing in other dimensions; and

iii. a plurality of magnetic or magnetically inducible particles in said chamber; and

b. a magnetic device, comprising;

i. means for generating a moving magnetic field; and

ii. means for retaining said chamber device in an orientation so that said moving magnetic field causes said particle to move in said chamber over a distance sufficient to effect mixing.

2. The system of claim 1, wherein in said chamber device, said chamber is part of a capillary passageway comprising an entry port in a surface of said housing, a pre-chamber passageway leading from said entry port to said capillary chamber, and a vent, wherein said vent is located in said chamber or in a post-chamber passageway that is connects said vent to said chamber.

3. The system of claim 1, wherein said magnetic device further comprises an optical detection system oriented to interrogate said chamber device at a location in said post-chamber passageway.

4. The system of claim 1, wherein said magnetic device comprises means for generating a magnetic field that imparts rotational motion to said particles.

5. The system of claim 1, wherein said magnetic device comprises means for generating a magnetic field that imparts linear motion to said particles.

6. The system of claim 5, wherein said magnetic means comprises collection means for causing said magnetic field to collect said particles into a sub-region of said y chamber.

7. The system of claim 6, wherein said chamber device further comprises a stop flow junction that prevents flow of a liquid in said chamber past said junction when said liquid is under the influence of solely capillary and gravitational forces and said linear motion is selected to cause a liquid in said capillary passageway to flow past said stop flow junction.

8. A capillary mixing device, comprising:

a. a liquid impervious housing;

b. an interior space in said housing comprising;

i. a chamber in said housing having capillary spacing in one dimension and non-capillary spacing in other dimensions; and

,

ii. first and second capillary passageways in said housing connected to said chamber; and

c. a plurality of magnetic or magnetically inducible particles in said chamber.

9. The device of claim 8, wherein substantially all of said particles are smaller than a magnetic domain.

10. The device of claim 8, wherein said capillary spacing is from 0.01 to 2 mm.

11. The device of claim 8, wherein said particles occupy from 1 to 5% of the volume of said chamber.

12. The device of claim 8, wherein said particles have a density of at least 4 g/cc.

13. The device of claim 8, wherein said particles comprise magnetite in a polymeric coating.

14. The device of claim 8, wherein said particle consist essentially of magnetite or barium ferrite.

15. A method of mixing in a capillary chamber, comprising:

a. adding a liquid to be mixed to a mixing device comprising;

i. a liquid impervious housing;

ii. a chamber in said housing having capillary spacing in one dimension and non-capillary spacing in other dimensions; and

iii. a plurality of magnetic or magnetically inducible particles in said chamber; and

b. generating a rotating magnetic field in said chamber.

16. The method of claim 15, wherein said magnetic field rotates at an angular velocity of from 400 to 3000 rpm.

17. The method of claim 15, wherein said magnetic field is generated by physically rotating a permanent magnet.

18. The method of claim 15, wherein the axis of said rotating magnetic passes through said chamber.

19. The method of claim 15, wherein said particles are present in said capillary chamber prior to adding said liquid to be mixed.

20. The method of claim 19, wherein said particles are present in a reagent composition soluble or dispersible in said liquid.

21. The method of claim 15, wherein said particles are introduced into said chamber concurrently with a liquid to be mixed in said capillary chamber.