Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
First Claim
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1. A bioelectrode comprising:
- a conducting surface;
a peroxidase adsorbed in a monolayer or close to a monolayer onto colloidal gold particles wherein the colloidal gold-adsorbed peroxidase is deposited on the conducting surface; and
an oxidase layer atop the deposited colloidal gold adsorbed peroxidase wherein direct electron transfer to the conducting surface occurs in the presence of an analyte when the bioelectrode is suitably coupled with a reference/counter electrode.
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Abstract
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytes depending on the oxidase employed.
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Citations
15 Claims
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1. A bioelectrode comprising:
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a conducting surface; a peroxidase adsorbed in a monolayer or close to a monolayer onto colloidal gold particles wherein the colloidal gold-adsorbed peroxidase is deposited on the conducting surface; and an oxidase layer atop the deposited colloidal gold adsorbed peroxidase wherein direct electron transfer to the conducting surface occurs in the presence of an analyte when the bioelectrode is suitably coupled with a reference/counter electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 12, 15)
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- 9. A bioelectrode for mediatorless electrochemical detection of glucose comprising a conducting surface having a first deposited layer of monolayer-coated colloidal gold-adsorbed horseradish peroxidase and a second deposited layer of colloidal gold-adsorbed glucose oxidase atop said first layer.
- 13. A process for preparing a bioelectrode comprising depositing a coating thickness of between 10 to 20 monolayers of a colloidal gold-adsorbed monolayer of peroxidase onto a conducting surface.
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