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Method for treating connective tissue disorders

  • US 5,226,914 A
  • Filed: 11/16/1990
  • Issued: 07/13/1993
  • Est. Priority Date: 11/16/1990
  • Status: Expired due to Term
First Claim
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1. A method for repairing connective tissue damage comprising:

  • a) providing culturally expanded purified human marrow-derived mesenchymal cells which have been isolated from a bone marrow specimen by adding the bone marrow specimen to a medium which contains factors which stimulate mesenchymal stem cell growth without differentiation and allows, when cultured, for selective adherence of only the mesenchymal stem cells to a substrate surface; and

    ,b) applying the culturally expanded purified human marrow-derived mesenchymal cells to an area of connective tissue damage under conditions suitable for differentiating the cells into connective tissue cells necessary for repair.

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