Vacuum clamped multi-sample filtration apparatus

• US 5,227,137 A
• Filed: 08/06/1992
• Issued: 07/13/1993
• Est. Priority Date: 04/04/1991
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Nicholson Precision Instruments, Inc.
• Inventors: White, Richard J., Monti, Patricia C., Nicholson, Michael K.
• Primary Examiner(s): Kryza, Lynn M.
• Application Number:
  US07/924,795
• Time in Patent Office: 341 Days
• Field of Search: 422/101, 422/102, 422/103, 422/58, 436/165, 436/178, 436/180, 436/809, 435/300, 435/301, 435/310, 435/311, 435/809, 210/406, 210/416.1, 210/455, 210/474, 210/477
• US Class Current: 422/534
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An apparatus for filtering multiple biological samples using a filter membrane is disclosed. The apparatus comprises a base plate, a well plate, a gasket, and a first vacuum member for vacuum-clamping the base plate, well plate, gasket and filter membrane together. The apparatus may further comprise a second vacuum member for drawing the biological samples from the well plate into contact with the filter membrane.

Claims

1. An apparatus for filtering a plurality of liquid samples using a filter membrane and for preventing cross-contamination by migration of the liquid samples along the filter membrane, the apparatus comprising:

(a) a well plate having a plurality of wells for receiving the liquid samples, said well plate having a substantially flat upper surface and a substantially flat lower surface, wherein each of said plurality of wells has a top opening in said upper surface and a bottom opening in said lower surface;

(b) a base plate having a substantially flat upper surface constructed and arranged to mate with said lower surface of said well plate;

(c) first vacuum means for vacuum-clamping said well plate to said base plate with the filter membrane disposed therebetween such that the filter membrane is held in sealable contact with said bottom openings of said plurality of wells of said well plate to preclude leakage of the liquid samples from said plurality of wells except through the filter membrane and to substantially preclude migration of the liquid samples between said wells along the filter membrane; and

(d) second vacuum means for drawing the liquid samples contained in said plurality of wells through the filter membrane, said second vacuum means being coupled to said bottom openings of said plurality of wells, through the filter membrane, when said first vacuum means clamps said base plate to said well plate.

2. The apparatus of claim 1, further comprising a flexible, resilient gasket disposed between said well plate and said base plate, said gasket isolating action of said first vacuum means from action of said second vacuum means.

3. The apparatus of claim 1, wherein said first vacuum means comprises a vacuum clamping area formed at a peripheral edge of said upper surface of said base plate and a peripheral edge of said lower surface of said well plate, said vacuum clamping area constructed and arranged to vacuum-clamp said well plate to said base plate when a vacuum is created in said vacuum clamping area.

4. The apparatus of claim 3, wherein said second vacuum means comprises a vacuum reservoir area formed at a central portion of said upper surface of said base plate, said vacuum reservoir area constructed and arranged to create a vacuum at said bottom openings of said plurality of wells of said well plate when said well plate is vacuum clamped to said base plate by said first vacuum means.

5. The apparatus of claim 4, further comprising:

election means for operating said second vacuum means independently of said first vacuum means.

6. The apparatus of claim 5, wherein said election means comprises a vacuum clamping valve disposed on said base plate and connectable to an external vacuum source to selectively create a vacuum in said vacuum clamping area and a vacuum sampling valve disposed on said base plate and connectable to an external vacuum source to selectively create a vacuum in said vacuum reservoir area.

7. The apparatus of claim 6, wherein said first vacuum means and said second vacuum means further comprise a flexible, resilient gasket disposed between said filter membrane and said base plate, said gasket isolating said vacuum clamping area, isolating said vacuum sampling area, and maintaining the filter membrane in sealable contact with each of said plurality of wells of said well plate.

8. An apparatus for filtering a plurality of liquid samples, the apparatus comprising:

(a) a well plate having a plurality of wells for receiving the liquid samples, said well plate having a substantially flat upper surface and a substantially flat lower surface, wherein each of said plurality of wells has a top opening in said upper surface and a bottom opening in said lower surface;

(b) a base plate having a substantially flat upper surface constructed and arranged to mate with said lower surface of said well plate;

(c) a filter membrane disposed between said lower surface of said well plate and said upper surface of said base plate;

(d) first vacuum means for vacuum-clamping said well plate to said base plate and for holding said filter membrane in sealable contact with said bottom openings of said plurality of wells of said well plate to substantially preclude leakage of the liquid samples from said plurality of wells except through said filter membrane and to substantially preclude migration of the liquid samples between said plurality of wells along said filter membrane; and

(e) second vacuum means for drawing the liquid samples contained in said plurality of wells through said filter membrane, said second vacuum means being coupled to said bottom openings of said plurality of wells, through said filter membrane, when said first vacuum means clamps said base plate to said well plate.

9. The apparatus of claim 8, further comprising a flexible, resilient gasket disposed between said filter membrane and said base plate, said gasket isolating action of said first vacuum means from action of said second vacuum means.

10. The apparatus of claim 8, wherein said first vacuum means comprises a vacuum clamping area formed at a peripheral edge of said upper surface of said base plate and a peripheral edge of said lower surface of said well plate, said vacuum clamping area constructed and arranged to vacuum-clamp said well plate to said base plate when a vacuum is created therein.

11. The apparatus of claim 10, wherein said second vacuum means comprises a vacuum reservoir area formed at a central portion of said upper surface of said base plate, said vacuum reservoir area constructed and arranged to create a vacuum at said bottom openings of said plurality of wells of said well plate when said well plate is vacuum clamped to said base plate by action of said first vacuum means.

12. The apparatus of claim 11, further comprising:

election means for operating said second vacuum means independently of said first vacuum means.

13. The apparatus of claim 12, wherein said election means comprises a vacuum clamping valve disposed on said base plate and connectable to an external vacuum source to selectively create a vacuum in said vacuum clamping area and a vacuum sampling valve disposed on said base plate and connectable to an external vacuum source to selectively create a vacuum in said vacuum reservoir area.

14. The apparatus of claim 13, wherein said first vacuum means and said second vacuum means further comprise a flexible, resilient gasket disposed between said filter membrane and said base plate, said gasket isolating said vacuum clamping area, isolating said vacuum sampling area, and maintaining said filter membrane in sealable contact with each of said plurality of wells of said well plate.

15. An apparatus for filtering a plurality of liquid samples, the apparatus comprising:

(a) a base plate having a substantially flat upper surface, said base plate including a first vacuum inlet, a second vacuum inlet, a vacuum clamping valve, and a vacuum sampling valve, said base plate partially defining a vacuum clamping area and a vacuum reservoir area, said first vacuum inlet being coupled to said vacuum clamping area through said vacuum clamping valve to selectively create a vacuum in said vacuum clamping area, said second vacuum inlet being coupled to said vacuum reservoir area through said vacuum sampling valve to selectively create a vacuum in said vacuum reservoir area;

(b) a well plate removably mounted on said base plate and having a substantially flat lower surface constructed and arranged to mate with said upper surface of said base plate when said well plate is mounted on said base late, said well plate having a plurality of sample wells, each of said plurality of sample wells including a top opening for receiving the liquid samples and a bottom opening in said lower surface in communication with said vacuum reservoir area of said base plate;

(c) a filter membrane, through which the liquid samples are to be filtered, disposed between said upper surface of said base plate and said lower surface of said well plate, said filter membrane being positioned between said bottom openings of said sample wells and said vacuum reservoir area of said base plate; and

(d) a flexible, resilient gasket disposed between said filter membrane and said upper surface of said base plate, said gasket isolating said vacuum clamping area from communication with said vacuum reservoir area and maintaining a seal between said lower surface of said well plate and said vacuum clamping area of said base plate so that said base plate is vacuum clamped to said well plate when a vacuum is created in said vacuum clamping area via said first vacuum inlet and said vacuum clamping valve, said gasket further maintaining said filter membrane in sealable contact with said bottom openings of said plurality of sample wells of said well plate to substantially preclude migration of the liquid samples between said wells along said filter membrane when the liquid samples are drawn through said filter membrane by action of a vacuum created in said vacuum reservoir area via said second vacuum inlet and said vacuum sampling valve.

16. The apparatus of claim 15, wherein said gasket includes first and second substantially parallel elastomeric surfaces and a plurality of well openings corresponding to respective ones of said bottom openings of said plurality of sample wells of said well plate.

17. The apparatus of claim 16, wherein said vacuum clamping area of said base plate includes a plurality of raised islands which extend upward from said upper surface of said base plate, and wherein said gasket includes a plurality of webs and bridges constructed and arranged to mate with respective ones of said islands.