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Method for microencapuslation of cells or tissue

  • US 5,227,298 A
  • Filed: 08/17/1990
  • Issued: 07/13/1993
  • Est. Priority Date: 08/17/1990
  • Status: Expired due to Term
First Claim
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1. A double-walled bead produced as a result of a method of encapsulating viable tissue or cells within a double-walled bead comprising:

  • (a) treating the tissue or cells, prior to encapsulation, with ultraviolet-B irradiation so as to immunosuppress them;

    (b) suspending the tissue or cells in an aqueous medium which is physiologically compatible with the tissue or cells and which contains a water soluble substance which(i) is physiologically compatible with the tissue or cells; and

    (ii) can be gelled to form a bead;

    (c) forming the suspension into droplets of a size sufficient to encapsulate the tissue or cells;

    (d) treating the droplets so as to form discrete, shape-retaining temporary capsules;

    (e) forming a permanent semipermeable membrane around the temporary capsules so as to obtain a single-walled bead encapsulating the tissue or cells; and

    (f) contacting the resulting single-walled bead with the water soluble substance under conditions such that a second membrane is formed so as to thereby obtain a double-walled bead encapsulating the tissue or cells.

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