Automatic multiple-sample multiple-reagent chemical analyzer

• US 5,229,074 A
• Filed: 12/09/1991
• Issued: 07/20/1993
• Est. Priority Date: 07/25/1988
• Status: Expired due to Term

• Current Assignee: Precision Systems, Inc. (Anschutz Company)
• Original Assignee: Precision Systems, Inc. (Anschutz Company)
• Inventors: Jones, Kermit W., Heath, Warren J., Bell, Charles A., Langner, Rene J.
• Primary Examiner(s): Woodard, Joye L.
• Application Number:
  US07/807,772
• Time in Patent Office: 589 Days
• Field of Search: 436/48, 73/864.21, 356/328, 422/64, 422/67
• US Class Current: 422/64
• CPC Class Codes: G01N 2035/00425 Heating or cooling means associated with pipettes or the like, e.g. for supplying sample/reagent at given temperature
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: Y10T 436/114165 with step of insertion or removal from test line

Abstract

An analyzer for chemical assays includes a turntable which supports sample containers and reagent containers within a cooled volume and test cells about the periphery of the cooled volume. A probe is carried by a rotating arm for withdrawing liquid from the containers and dispensing the liquid. Fixed stations are also provided in the path of the probe. One such station serves to both wash the tip of the probe and to receive samples for flow-through analysis. Heaters associated with the probe and with the test cells increase the rate of reaction. Curved walls of test cell segments match the optics of an optical analyzer so that light from the analyzer lamp is generally orthogonal to the curved surfaces. The light voltage of the lamp may be controlled as a function of lamp wavelength required for particular assays. The system provides multiple-point calibration and automatic dilution of samples.

Claims

1. An analyzer system for mixing samples and reagents and performing an optical analysis thereof, the analyzer comprising:

a turntable which supports a plurality of sample containers containing sample liquid in a circular array, each of the plurality of sample containers being removable from the turntable independently from each other, and a plurality of larger reagent containers supported by the turntable for containing reagent liquid in a circular array, each of the plurality of larger reagent containers being removable from the turntable independently from each other and independently from the sample containers;

a circular array of removable transparent test cells surrounding the plurality of reagent containers and sample containers, the test cells being supported on the turntable and being removable from the turntable independently from the plurality of sample containers and reagent containers;

an optical analyzer positioned adjacent to the turntable which illuminates a selected test cell on the turntable and detects light from the cell to provide a signal representative of a property of liquid in the test cell;

a translatable probe which withdraws liquid from selected ones of the sample containers and the reagent containers and dispenses withdrawn liquid into a selected test cell to mix liquid from the sample and reagent containers, the probe being selectively positionable over all sample and reagent containers and all test cells by translation of the probe and rotation of the turntable; and

a programmable controller and drive assembly, the controller being programmed for rotating the turntable, translating the probe and causing the probe to withdraw and dispense sample liquid and reagent liquid to mix reagent and samples in test cells and perform tests as programmed for individual samples, the controller being programmed to dispense liquid from a common reagent container into plural test cells and liquid from a common sample container into plural test cells.

2. An analyzer system as claimed in claim 1 further comprising a plurality of fixed stations adjacent to the turntable and positioned along a path of the probe away from the turntable.

3. An analyzer system as claimed in claim 1 wherein the probe is positioned on the end of a rotating arm.

4. An analyzer system as claimed in claim 3 wherein the arm is mounted for vertical translation.

5. An analyzer system as claimed in claim 4 further comprising a detector for detecting the vertical height of the probe and a level detector for detecting the position of the probe relative to liquid within a container.

6. An analyzer system as claimed in claim 3 further comprising optical encoders which indicate angular positions of the turntable and of the rotating arm about axes of rotation.

7. An analyzer system as claimed in claim 1 further comprising a heater which heats the test cells, and a walled barrier between the test cells and the sample and reagent containers to provide thermal isolation.

8. An analyzer system as claimed in claim 1 further comprising a heater which heats liquid drawn into the probe before the liquid is dispensed from the probe.

9. An analyzer system as claimed in claim 1 further comprising a fixed probe washer station adjacent to the turntable along a path of the probe.

10. An analyzer system as claimed in claim 9 wherein the probe washer comprises a well into which the probe may be positioned and a fluid inlet for introducing washer fluid into the well, the washer further comprising a conduit at the base of the well into which the tip of the probe may be positioned, the conduit being coupled to an analyzer such that, after washing of the end of the probe, the probe may be positioned to inject liquid therefrom to the analyzer.

11. An analyzer system as claimed in claim 1 wherein the turntable comprises a first tray for carrying the sample containers and the reagent containers in the circular array and a second tray positionable over and removable from the first tray and coupled to rotate therewith for carrying a second circular array of sample containers.

12. An analyzer system as claimed in claim 1 wherein the test cells comprise inner and outer transparent walls having inner and outer surfaces which are curved such that light from the optical analyzer illuminating a test cell strikes the test cell generally orthogonally to the inner and outer surfaces of the inner and outer test cell walls.

13. An analyzer system as claimed in claim 1 wherein the test cells comprise circular segments of multiple cells, each circular segment comprising inner and outer curved walls joined by cell dividing walls for receiving the light from the optical analyzer.

14. An analyzer system as claimed in claim 1, wherein the optical analyzer further comprises a lamp of multiple wavelengths which illuminates the test cell and a light controller for controlling a variable operating level of electrical input to the lamp as a function of wavelength of interest in optical analysis.

15. An analyzer system as claimed in claim 14 wherein the test cells comprise inner and outer transparent walls having inner and outer surfaces which are curved such that light from the lamp illuminating the test cell strikes the test cell generally orthogonally to the test cell walls.

16. An analyzer system as claimed in claim 15, wherein the optical analyzer further comprises a grating which receives light from the test cell and directs light of different wavelengths to different portions of a detector array.

17. An analyzer system as claimed in claim 1, wherein the optical analyzer further comprises a grating which receives light from the test cell and directs light of different wavelengths to different portions of a detector array.

18. An analyzer system as claimed in claim 1 wherein the controller includes means for causing short, horizontal, oscillating relative movement between the probe and the turntable, with the probe positioned in a test cell, in order to mix liquid in the test cell.

19. An analyzer system as claimed in claim 18 wherein the controller causes movement of the turntable to cause the short relative movement.

20. An analyzer system as claimed in claim 19 wherein the controller causes movement of the probe to cause the short relative movement.

21. An analyzer system as claimed in claim 1, wherein the controller comprises means for performing an optical analysis of standard samples from plural predetermined sample containers on the turntable to define a calibration curve and to calibrate tests of liquid from other sample containers with the defined calibration curve.

22. An analyzer system as claimed in claim 1 wherein the controller comprises means for controlling automatic dilution of samples and providing an analysis output as a function of the amount of dilution.

23. An analyzer system as claimed in claim 22 wherein the controlling means controls automatic dilution of samples determined by the controller to provide a signal from the optical analyzer which is beyond a calibrated signal level.

24. An analyzer system as claimed in claim 22 wherein the controlling means may be caused to automatically dilute a sample in response to operator instructions.

25. An analyzer system as claimed in claim 1, wherein the controller comprises means responsive to bar code inputs for identifying samples and bar code inputs which determine particular assay procedures for the samples.

26. An analyzer system as claimed in claim 25 further comprising means for printing a sheet of labels, each label identifying in human readable and machine-readable bar code formats, the source of a test sample.

27. An analyzer system as claimed in claim 1 further comprising means for cooling the sample containers and reagent containers, and means for thermally isolating the test cells from the sample and reagent containers.

28. An analyzer system as claimed in claim 1 wherein the test cells are mounted together as a plurality of segments forming a full array of transparent test cells surrounding the plurality of reagent and sample containers.

29. An analyzer system as claimed in claim 28 further comprising a cooler for cooling the sample and reagent containers supported on the turntable, and means for thermally isolating the test cells from the sample and reagent containers.

30. An analyzer system as claimed in claim 1 further comprising motors coupled to the turntable and translatable probe through shock absorbing couplings for driving the turntable and the translatable probe.

31. An analyzer system for mixing samples and reagents and performing an optical analysis thereof comprising:

a turntable which supports a plurality of independently removable sample containers containing sample liquid, in a circular array and a plurality of independently removable larger reagent containers, each containing reagent liquid for mixing with sample liquid from any of the sample containers, about the periphery in the circular array;

a peripheral array of removable transparent test cells supported on the turntable and surrounding the sample and reagent containers, the test cells being thermally isolated from the sample and reagent containers by an isolating means, and the test cells being removable independently of all reagent and sample containers;

an optical analyzer which illuminates a selected test cell and detects light from the cell to provide a signal representative of a property of liquid in the test cell;

a rotating arm which carries a probe for withdrawing liquid from the sample containers and the reagent containers and dispensing withdrawn liquid into the test cells to mix liquid from the sample and reagent containers, the probe being selectively positionable over all sample and reagent containers and all test cells by rotation of the arm and rotation of the turntable;

a probe washing station adjacent to the turntable along a curved path at the radius of the probe on the arm;

a plurality of stationary containers adjacent to the turntable positioned along the curved path at the radius of the probe on the arm; and

a programmable controller and drive assembly for rotating the turntable, translating the probe and causing the probe to withdraw and dispense sample liquid and reagent liquid to mix reagent and samples in test cells and perform tests as programmed for individual samples.

32. An analyzer system as claimed in claim 31 wherein the probe washing station comprises, each comprise a well into which the probe may be positioned and a fluid inlet for introducing washer fluid into the well, the washing station further comprising a conduit at the base of the well into which the tip of the probe may be positioned, the conduit being coupled to an analyzer such that, after washing of the end of the probe, the probe may be positioned to inject liquid therefrom to the analyzer.

33. An analyzer system for mixing samples and reagents and performing an optical analysis thereof comprising:

a turntable which supports a plurality of sample containers containing sample liquid, in a circular array and a plurality of larger reagent containers supported by the turntable containing reagent liquid for mixing with sample liquid from any of the sample containers in the circular array, each of the plurality of larger reagent containers being removable from the turntable independently from each other and independently from the sample containers;

a tray positionable over and removable from the turntable and coupled to rotate therewith, the tray carrying shorter sample containers;

a peripheral array of removable transparent test cells supported on the turntable and surrounding the sample and reagent containers, the test cells being thermally isolated from the sample and reagent containers by an isolating means, and the test cells being removable independently of all other reagent and sample containers;

an optical analyzer which illuminates a selected test cell and detects light from the cell to provide a signal representative of a property of liquid in the test cell;

a translatable probe which withdraws liquid from the selected ones of the sample containers and the reagent containers and dispenses withdrawn liquid into a selected test cell to mix liquid from the sample and reagent containers, the probe being selectively positionable over all sample and reagent containers and all test cells by translation of the probe along a path and rotation of the turntable; and

a programmable controller and drive assembly for rotating the turntable, translating the probe and causing the probe to withdraw and dispense sample liquid and reagent liquid to mix reagent and samples in test cells and perform tests as programmed for individual samples.

34. An analyzer system for mixing samples and reagents and performing an optical analysis thereof, the analyzer comprising:

a turntable which supports a plurality of independently removable sample containers, containing sample liquid, in a circular array and a plurality of independently larger reagent containers, each containing reagent liquid for mixing with sample liquid from any of the sample containers, about the periphery of the circular array;

a cooler for cooling the sample and reagent containers supported on the turntable;

a peripheral array of transparent test cells supported on the turntable and surrounding but being thermally isolated from the sample and reagent containers by an isolating means, the test cells being removable independently of all other containers;

an optical analyzer positioned adjacent to the turntable which illuminates a selected test cell and detects light from the cell to provide a signal representative of a property of liquid in the test cell;

a rotating arm which carries a probe for withdrawing liquid from the sample and reagent containers and dispensing withdrawn liquid into the test cells to mix liquid from the sample and reagent containers, the probe being selectively positionable over all sample and reagent containers and all test cells by rotation of the arm and rotation of the turntable;

a probe washing station adjacent to the turntable along a curved path at the radius of the probe on the arm;

a plurality of stationary containers adjacent to the turntable positioned beyond the probe washing station along the curved path at the radius of the probe on the arm; and

a programmable controller and drive assembly for rotating the turntable, translating the probe and causing the probe to withdraw and dispense sample liquid and reagent liquid to mix reagent and samples in test cells and perform tests as programmed for individual samples.

35. An analyzer system as claimed in claim 34 further comprising a heater placed adjacent to the turntable for heating the test cells.

36. An analyzer system as claimed in claim 34 further comprising a heater which heats liquid drawn into the probe before the liquid is dispensed from the probe.

37. An analyzer system as claimed in claim 34 wherein the turntable comprises a first tray for carrying the sample containers and the reagent containers in the circular array and a second tray positionable over and removable from the first tray and coupled to rotate therewith for carrying a second circular array of sample containers.

38. An analyzer system as claimed in claim 34 wherein the test cells comprise inner and outer transparent walls having inner and outer surfaces which are curved such that light from the optical analyzer illuminating the test cell strikes the test cell generally orthogonally to the inner and outer surfaces of the inner and outer test cell walls.

39. An analyzer system for mixing samples and reagents and performing an optical analysis thereof, the analyzer comprising:

a turntable which supports a plurality of sample containers containing sample liquid in a circular array, each of the plurality of sample containers being removable from the turntable independently from each other, and a plurality of larger reagent containers supported by the turntable for containing reagent liquid for mixing with sample liquid from any of the sample containers in a circular array;

a circular array of removable transparent test cells surrounding the plurality of reagent and sample containers, the test cells being supported on the turntable and being independently removable from the plurality of sample containers and reagent containers;

an optical analyzer which illuminates a selected test cell on the turntable and detects light from the cell to provide a signal representative of a property of liquid in the test cell;

a translatable probe which withdraws liquid from selected ones of the sample containers and the reagent containers and dispenses withdrawn liquid into a selected test cell to mix liquid from the sample and reagent containers, the probe being selectively positionable over all sample and reagent containers and all test cells by translation of the probe and rotation of the turntable; and

a programmable controller and drive assembly for rotating the turntable, translating the probe and causing the probe to withdraw and dispense sample liquid and reagent liquid to mix reagent and samples in test cells, perform tests as programmed for individual samples, and causing short, horizontal, oscillating relative movement between the probe and the turntable, wherein the probe and turntable each have short relative movement.