Process for preparing a microtiter tray for immunometric determinations

• US 5,229,163 A
• Filed: 04/26/1991
• Issued: 07/20/1993
• Est. Priority Date: 12/21/1989
• Status: Expired due to Fees

• Current Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Fox, William A.
• Primary Examiner(s): Lusigan, Michael
• Assistant Examiner(s): DUDASH, DIANA L
• Application Number:
  US07/691,758
• Time in Patent Office: 816 Days
• Field of Search: 427/2, 427/5, 427/43.1, 427/444, 427/154, 427/230, 427/300, 427/282, 427/4, 427/533, 427/536, 427/539, 427/540, 427/569, 427/580, 356/246, 422/102
• US Class Current: 204/169
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: G01N 2035/00168 Manufacturing or preparing test elements
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/56977 HLA or MHC typing

Abstract

A microtiter plate containing a plurality of reaction wells for conducting immunogenic reactions the bottom wall of the reaction well has an inner surface which is substantially hydrophilic, and the side wall of the reaction well has an inner surface which is substantially hydrophobic and a process for producing said trays.

Claims

1. A process for producing a tray for carrying out immunogenic reactions to determine the presence of a component of an antibody antigen reaction in a biological fluid sample through the use of reagents comprising:

(a) providing a plastic plate member formed from a hydrophobic plastic with a top and bottom surfaces, the top surface of said plate member defining a plurality of spaced apart wells in said top surface, with said wells having a bottom and side wall the inner surfaces of which being configured and adapted to hold and receive a biological fluid and reagents for conducting an immunogenic reactor; and

(b) selectively treating the inner surface of said bottom wall of the wells to render said inner surface of said bottom wall hydrophilic without affecting the hydrophobic nature of the inner surface of the side wall.

2. The process of claim 1 wherein the inner surface of the bottom wall is rendered hydrophilic by selectively exposing only the inner surface of the bottom wall to oxidation while shielding the inner surface of the side wall from said exposure.

3. The process of claim 2 wherein oxidation of the inner surface of the bottom wall is caused by treatment with electrons discharged by an electrode discharge means.

4. The process of claim 3 wherein the shield is a mat formed from a material which is impervious to the electrons discharged by an electrode discharge means, said mat having spaced apart apertures, said apertures being spaced upon said mat so that when said mat is placed upon the top surface of said plate member, the apertures coincide with the bottom walls of said wells with the surface area of each of said apertures being dimensioned to the same surface area of bottom wall of said wells so that only the surface area of the bottom walls of said well is exposed to the electrons discharged by the electrode discharge means.

5. The process of claim 4 comprising shielding the inner surfaces of the side wall by providing the mat with flange member surrounding the aperature and projecting to the inner surface of the bottom wall of said well to cover the inner surface of said wall.

6. The process of claim 5 wherein said material is rubber.