Process for producing ethanol

• US 5,231,017 A
• Filed: 05/17/1991
• Issued: 07/27/1993
• Est. Priority Date: 05/17/1991
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Solvay Enzymes Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Fish, John J., Lantero, Oreste J.
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US07/701,871
• Time in Patent Office: 802 Days
• Field of Search: 435/161, 435/163, 435/162, 435/165, 435/225, 435/93, 426/11, 426/16, 426/29, 425/225
• US Class Current: 435/161
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The invention relates to a process for producing ethanol from raw materials, that contain fermentable sugars or constituents which can be converted into sugars, comprising the steps of:

a-liquefaction of the raw materials in the presence of an alpha-amylase for obtaining liquefied mash,

b-saccharification of the liquefied mash in the presence of a glucoamylase for obtaining hydrolyzed starch and sugars,

c-fermentation of the hydrolyzed starch and sugars by yeast for obtaining ethanol, and

d-recovering alcohol,

a protease being introduced in the liquefied mash during the saccharification and/or in the hydrolyzed starch and sugars during the fermentation.

The invention relates also to a composition containing a glucoamylase and an acid fungal protease.

Claims

1. A process for producing ethanol from raw materials containing a high dry solid mash level having fermentable sugars or constituents which can be converted into sugars, comprising the steps of:

a. liquefaction of the raw materials in the presence of an alpha amylase to obtain liquefied mash;

b. saccharification of the liquefied mash in the presence of a glucoamylase to obtain hydrolysed starch and sugars;

c. fermentation of the hydrolysed starch and sugars by yeast to obtain ethanol; and

d. recovering the obtained ethanol, wherein an acid fungal protease is introduced to the liquefied mash during the saccharification and/or to the hydrolysed starch and sugars during the fermentation, thereby increasing the rate of production of ethanol as compared to a substantially similar process conducted without the introduction of the protease.

2. The process according to claim 1 wherein the fungal protease is a protease from Aspergillus niger.

3. The process according to claim 1 wherein the saccharification and the fermentation steps are carried out simultaneously.

4. The process according to claim 1 wherein the raw materials are whole ground corns, cobs, corns, grains, milo or cereals and their mixtures.

5. The process according to claim 1 wherein the glucoamylase and the acid fungal protease are introduced as a mixture or a composition and used as a single addition.

6. The process according to claim 1 wherein the liquefied mash contains a high dry solids mash level.

7. A composition containing a glucoamylase and an acid fungal protease used in a process according to claim 1.

8. The composition according to claim 7 wherein the glucoamylase is derived from Aspergillus niger and the acid fungal protease is derived from Aspergillus niger.

9. The process according to claim 1, wherein the total yield of ethanol is increased.

10. The process according to claim 1, wherein the raw materials comprise between 20% and 40% of the high dry solid mash.

11. The process according to claim 1, wherein the raw materials comprise between 25% and 35% of the high dry solid mash.