High molecular weight DNA compositions for use in electrophoresis of large nucleic acids
First Claim
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1. A composition of matter comprising:
- (a) a first DNA species of about x Mb in length;
(b) a second DNA species of about 2x Mb in length; and
(c) a third DNA species of about 3x Mb in length.wherein x is a constant greater than about 0.05, and wherein the composition is capable of generating a series of discrete bands when subjected to gel electrophoresis wherein the DNA in any one of such bands differs in length from the DNA in any other of the band by an integral multiple of about x.
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Abstract
The invention provides high molecular weight DNA length standards useful for sizing very long DNA molecules and methods for producing such standards. In a preferred embodiment, the invention provides a mixture of T4 DNA concatemers that forms a DNA "ladder" having rungs differing by at least 0.17 Mb (Megabases) in length upon gel electrophoresis and methods for producing and using such compositions.
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11 Claims
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1. A composition of matter comprising:
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(a) a first DNA species of about x Mb in length; (b) a second DNA species of about 2x Mb in length; and (c) a third DNA species of about 3x Mb in length. wherein x is a constant greater than about 0.05, and wherein the composition is capable of generating a series of discrete bands when subjected to gel electrophoresis wherein the DNA in any one of such bands differs in length from the DNA in any other of the band by an integral multiple of about x. - View Dependent Claims (3)
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2. A composition of matter consisting essentially of:
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(a) a first DNA species of about x Mb in length; (b) a second DNA species of about 2x Mb in length; and (c) a third DNA species of about 3x Mb in length; wherein x is a constant greater than about 0.05.
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4. A composition of matter comprising a mixture of monomers and oligomers of a selected DNA molecule, and wherein the monomers of the molecule are at least about x Mb in length and x≧
- about 0.05.
- View Dependent Claims (5, 6, 7, 8, 9, 10, 11)
Specification