Enzyme sensor

• US 5,236,567 A
• Filed: 05/24/1990
• Issued: 08/17/1993
• Est. Priority Date: 05/31/1989
• Status: Expired due to Fees

• Current Assignee: Nakano Vinegar Co., Ltd.
• Original Assignee: Nakano Vinegar Co., Ltd.
• Inventors: Nanba, Akira, Okumura, Hajime, Fukaya, Masahiro, Kawamura, Yoshiya
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US07/528,088
• Time in Patent Office: 1,181 Days
• Field of Search: 204/403, 204/153.12, 204/416, 204/418, 204/419, 435/817, 435/288, 435/291, 436/518, 436/806
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

An enzyme sensor, which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises, as electrode components, an enzyme and/or an enzyme-containing substance and mediator. The enzyme sensor is useful in analysis, such as the analysis of compounds in foods or components in the living body, the diagnosis of diseases and the control of reaction processes. The preparation of the enzyme-modified electrode is also described.

Claims

1. An enzyme sensor which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises, as electrode components, a combination of (i) an enzyme or an enzyme-containing substance or an enzyme and an enzyme-containing substance and (ii) a mediator, wherein the enzyme and mediator are, respectively:

aldehyde dehydrogenase and p-benzoquinone;

fructose dehydrogenase and dimethylferrocene;

sorbitol dehydrogenase and dimethyl ferrocene;

glycerol dehydrogenase and potassium ferricyanide;

or alcohol dehydrogenase and a complex of potassium ferricyanide and a cationic surface-active agent.

2. A sensor according to claim 1, wherein the enzyme-containing substance is selected from the group consisting of cells of a microorganism producing said enzyme, a culture medium and disrupted cells of a microorganism producing said enzyme, fractionation components of disrupted cells of a microorganism producing said enzyme, cellular extracts of disrupted cells of a microorganism producing said enzyme, cell membrane fraction of disrupted cells of a microorganism producing said enzyme and a crude enzyme from disrupted cells of a microorganism producing said enzyme.

3. A sensor according to any one of claims 1 and 2, wherein the electrode components contain carbon or graphite.

4. A measuring apparatus which comprises a reaction chamber, a constant voltage power supply part, a current voltage converting part, and an amplifier part, said reaction chamber being equipped with a sample injection port, an electrolyte inlet, a waste liquid outlet, a stirring device, a counter electrode, and an enzyme-modified electrode, wherein said enzyme-modified electrode comprises, as electrode components, (i) an enzyme or an enzyme-containing substance or an enzyme and an enzyme-containing substance and (iii) a mediator.