End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
First Claim
1. Polyacrylamide supports to which RNA or DNA oligonucleotides are covalently attached via a thioether linkage, wherein:
- at least about 95% of the oligonucleotides are linked to the carboxamide side groups of the support at the 5'"'"'-ends of the oligonucleotides;
the polyacrylamide supports have a molecular weight exclusion limit of at least about 2×
107 daltons; and
the supports to which the oligonucleotides are covalently attached via a thioether linkage are selected from the group consisting of supports represented by the formulae;
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Abstract
This invention concerns methods and means for covalent attachment of oligonucleotides to solid supports substantially at their 5'"'"'-ends. According to the invention thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports.
In a further aspect, this invention relates to bromoacetyl-oligonucleotides that may be immobilized on thiol-polyacrylamide solid supports, thiol-oligonucleotides immobilized on bromoacetyl-derivatized polyacrylamide supports as well as to methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats.
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Citations
5 Claims
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1. Polyacrylamide supports to which RNA or DNA oligonucleotides are covalently attached via a thioether linkage, wherein:
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at least about 95% of the oligonucleotides are linked to the carboxamide side groups of the support at the 5'"'"'-ends of the oligonucleotides; the polyacrylamide supports have a molecular weight exclusion limit of at least about 2×
107 daltons; andthe supports to which the oligonucleotides are covalently attached via a thioether linkage are selected from the group consisting of supports represented by the formulae;
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5. Oligonucleotides derivatized at their 5'"'"'-terminal phosphate group with bromoacetyl group and having the following formula:
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Specification