Solid surface coated with a hydrophilic biopolymer-repellent outer layer and method of making such a surface

• US 5,240,994 A
• Filed: 09/13/1991
• Issued: 08/31/1993
• Est. Priority Date: 10/22/1990
• Status: Expired due to Fees

• Current Assignee: Berol Novel AB
• Original Assignee: Berol Novel AB
• Inventors: Brink, Carina, Osterberg, Eva, Holmberg, Krister
• Primary Examiner(s): Kight, III, John
• Assistant Examiner(s): Mullis, Jeffrey Culpepper
• Application Number:
  US07/759,019
• Time in Patent Office: 718 Days
• Field of Search: 525/57, 525/60, 525/54.2, 525/54.24, 525/54.26, 525/54.31, 525/54.21, 525/54.23
• US Class Current: 525/54.2
• CPC Class Codes: A61L 33/068 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds A61L33/062 takes precedence
• CPC Class Codes: C08G 81/00 Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers involving only carbon-to-carbon unsaturated bond reactions C08F299/00 ; polyester-amides C08G69/44; polyester-imides C08G73/16; polyamides-imides C08G73/14; block- or graft polymers containing polysiloxane sequences C08G77/42
• CPC Class Codes: C08J 2479/00 Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2461/00 - C08J2477/00
• CPC Class Codes: C08J 7/0427 with only one layer of a composition containing a polymer binder with more layers C08J7/042
• CPC Class Codes: C08J 7/056 Forming hydrophilic coatings
• CPC Class Codes: C08L 1/26 Cellulose ethers
• CPC Class Codes: C08L 71/00 Compositions of polyethers obtained by reactions forming an ether link in the main chain of polyacetals C08L59/00; of epoxy resins C08L63/00; of polythioether-ethers C08L81/02; of polyether-sulfones C08L81/06; Compositions of derivatives of such polymers
• CPC Class Codes: C08L 79/02 Polyamines

Abstract

A solid surface having anionic groups capable of reacting with a polyethylene imine substituted at least in part by at least 50% by weight of a hydrophilic nonionic polymer having a molecular weight within the range from about 400 to about 200,000 is provided with a hydrophilic biopolymer-repellent outer surface layer thereof by reacting such a polyethylene imine substituted by nonionic hydrophilic polymer with anionic groups on the solid surface, in an amount sufficient to produce biopolymer-repellent solid surface, and accordingly having low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction.

Claims

1. A process for preparing biopolymer-repellent solid surfaces having low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction and useful in biochemical applications, comprising reacting a solid surface having anionic groups capable of reacting with ammonium groups, with a polyethylene imine substituted at least in part by at least 50% by weight of nonionic hydrophilic polymer thereby linking the polyethylene imine/hydrophilic polymer to the surface via anionic groups thereof, and forming a hydrophilic outer surface layer repellent to biopolymer and having low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction.

2. A process according to claim 1 in which the nonionic hydrophilic polymer side chains are at least partly hydrophilic polyalkylene glycol chains deriving from at least one of ethylene oxide, propylene oxide, butylene oxide, and tetrahydrofuran.

3. A process according to claim 1 in which the nonionic hydrophilic polymer side chains are derived from water-soluble nonionic cellulose ethers.

4. A process according to claim 1 in which the solid surface is selected from polystyrene, polyvinyl chloride and polyethylene.

5. A biopolymer-repellent solid surface useful in biochemical applications, and having low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction, the solid surface having a hydrophilic outer surface layer comprising polyethylene imine which is bonded to the solid surface via anionic groups of the surface reacted with ammonium groups thereof, and which is also linked via groups reactive with amino groups of the polyethylene imine to hydrophilic nonionic polymer in an amount of at least 50% by weight of the polyethylene imine.

6. A biopolymer-repellent solid surface according to claim 5 in which the hydrophilic nonionic polymer comprises chains of polyalkylene glycol from at least one of ethylene oxide, polypropylene oxide, butylene oxide, and tetrahydrofuran, distributed randomly or in blocks.

7. A biopolymer-repellent solid surface according to claim 5 in which the solid surface is selected from polystyrene, polyvinyl chloride, and polyethylene.