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Method, system and reagents for DNA sequencing

  • US 5,242,796 A
  • Filed: 10/22/1991
  • Issued: 09/07/1993
  • Est. Priority Date: 07/02/1986
  • Status: Expired due to Term
First Claim
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1. A compound having the structure ##STR22## wherein X is H, NH2 or halogen, andY is H, halogen, OH, or NH2, orX=Y=OH;

  • B is uracil, cytosine, 7-deazaadenine, 7-deazaguanine or 7-deazahypoxanthine where the pyrimidines are linked to the sugar moiety through the N1 position, the purines and the deazapurines are linked to the sugar moiety through the N9 position (purine numbering);

    A is a fluorophore having the structure ##STR23## wherein n=2 or 3, and R1 and R2 are H, lower alkyl, halo, lower alkoxy, and cyano;

    and the dotted line represents a linker and optional spacer joining B and A provided that if B is a pyrimidine the attachment is to the 5-position of that pyrimidine and if B is a deazapurine, the attachment is to the 7-position of that deazapurine (purine numbering).

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