Method, system and reagents for DNA sequencing
First Claim
1. A compound having the structure ##STR22## wherein X is H, NH2 or halogen, andY is H, halogen, OH, or NH2, orX=Y=OH;
- B is uracil, cytosine, 7-deazaadenine, 7-deazaguanine or 7-deazahypoxanthine where the pyrimidines are linked to the sugar moiety through the N1 position, the purines and the deazapurines are linked to the sugar moiety through the N9 position (purine numbering);
A is a fluorophore having the structure ##STR23## wherein n=2 or 3, and R1 and R2 are H, lower alkyl, halo, lower alkoxy, and cyano;
and the dotted line represents a linker and optional spacer joining B and A provided that if B is a pyrimidine the attachment is to the 5-position of that pyrimidine and if B is a deazapurine, the attachment is to the 7-position of that deazapurine (purine numbering).
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Abstract
A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments.
The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3 H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method.
129 Citations
5 Claims
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1. A compound having the structure ##STR22## wherein X is H, NH2 or halogen, and
Y is H, halogen, OH, or NH2, or X=Y=OH; -
B is uracil, cytosine, 7-deazaadenine, 7-deazaguanine or 7-deazahypoxanthine where the pyrimidines are linked to the sugar moiety through the N1 position, the purines and the deazapurines are linked to the sugar moiety through the N9 position (purine numbering); A is a fluorophore having the structure ##STR23## wherein n=2 or 3, and R1 and R2 are H, lower alkyl, halo, lower alkoxy, and cyano; and the dotted line represents a linker and optional spacer joining B and A provided that if B is a pyrimidine the attachment is to the 5-position of that pyrimidine and if B is a deazapurine, the attachment is to the 7-position of that deazapurine (purine numbering).
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2. A compound having the structure ##STR24## wherein R1 and R2 are H or CH3.
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3. A compound having the structure ##STR25## wherein R1 and R2 are H or CH3.
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4. A compound having the structure ##STR26## wherein R1 and R2 are H or CH3.
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5. A compound having the structure ##STR27## wherein R1 and R2 are H or CH3.
Specification