Glycosylated haemoglobin assay
First Claim
1. A method of assessing glycosylated haemoglobin in a sample containing both glycosylated and non-glycosylated haemoglobin in solution, said method comprising the steps of:
- (a) contacting the sample solution with signal-forming molecules comprising a conjugate of one or more dihydroxyboryl residues or salts thereof, linked to a signal-forming label, to form a reaction mixture containing glycosylated haemoglobin having said signal-forming molecule bound thereto;
(b) precipitating from said reaction mixture by means of a non-immobilized precipitating agent glycosylated haemoglobin having said signal-forming molecule bound thereto, glycosylated haemoglobin and non-glycosylated haemoglobin;
(c) separating said precipitate from said reaction mixture; and
(d) assessing said signal-forming molecules which are bound to the separated haemoglobin, and assessing the separated glycosylated and non-glycosylated haemoglobin.
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Abstract
A method of assessing glycosylated haemoglobin in a sample, wherein the method comprises the steps of (a) contacting the sample with signal-forming molecules comprising a conjugate of one or more dihydroxyboryl residues or salts thereof, linked to a signal-forming label; (b) separating by selective precipitation from a homogenous solution, glycosylated and non-glycosylated haemoglobin and any molecules bound thereto, from the reaction mixture of step (a) above; and (c) assessing signal-forming molecules selected from the group consisting of signal-forming molecules which have bound to the separated haemoglobin, and non-haemoglobin bound signal-forming molecules. Steps (a) and (b) may be performed simultaneously or sequentially. The sample may optionally be haemolyzed to liberate any cell bound haemoglobin. The invention also comprises an analytical test kit for use in accordance with the method of the invention. The new assay of the invention is particularly useful for the in vitro diagnosis and monitoring of diabetes mellitus.
58 Citations
16 Claims
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1. A method of assessing glycosylated haemoglobin in a sample containing both glycosylated and non-glycosylated haemoglobin in solution, said method comprising the steps of:
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(a) contacting the sample solution with signal-forming molecules comprising a conjugate of one or more dihydroxyboryl residues or salts thereof, linked to a signal-forming label, to form a reaction mixture containing glycosylated haemoglobin having said signal-forming molecule bound thereto; (b) precipitating from said reaction mixture by means of a non-immobilized precipitating agent glycosylated haemoglobin having said signal-forming molecule bound thereto, glycosylated haemoglobin and non-glycosylated haemoglobin; (c) separating said precipitate from said reaction mixture; and (d) assessing said signal-forming molecules which are bound to the separated haemoglobin, and assessing the separated glycosylated and non-glycosylated haemoglobin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification