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Device for performing solid-phase diagnostic assay

  • US 5,244,630 A
  • Filed: 10/23/1989
  • Issued: 09/14/1993
  • Est. Priority Date: 04/22/1988
  • Status: Expired due to Term
First Claim
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1. A device suitable for performing a plurality of automated solid-phase diagnostic assays, said device comprising a plurality of elements each comprising:

  • a sample well for receiving a sample and reagents for forming a reaction mixture which produces a detectable reaction product corresponding to an analyte in said sample;

    a read well positioned adjacent to said sample well comprising;

    (a) an entrance port and holding means for receiving and holding said reaction mixture from said sample well;

    (b) a fibrous matrix for immobilizing said reaction product for detection thereof, said fibrous matrix positioned below and in fluid communication with said holding means, and having an average separation of fibers to allow free flow of said reaction mixture;

    (c) an absorbent material positioned below and in fluid communication with said fibrous matrix to absorb any sample or assay reagents that flow through said fibrous matrix;

    means for venting air entrapped in the absorbent material and displaced by fluids absorbed therein; and

    passage means communicating between said sample well and said read well such that when wash fluid is injected into said sample well said reaction mixture is washed from said sample well through said passage means into said read well.

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