Analytical method
First Claim
1. A method for indicating the presence of an analyte component in a fluid mixture containing the analyte comprising:
- contacting a selective dissolving means with the fluid mixture to dissolve analyte molecules from the fluid mixture, to provide the analyte molecules in the dissolved or solution phase in the selective dissolving means, and to provide a controlled environment in which the analyte molecules are exposed to a source radiant energy to directly excite the analyte molecules and raise the energy level of the analyte molecules to an excited energy state and a controlled environment in which the excited analyte molecules return to a lower energy level and emit radiant energy from the analyte molecules, said selective dissolving means being substantially transparent to the source radiant energy and to the emitted radiant energy;
directing source radiant energy, which is suitable for directly exciting the analyte molecules to an elevated energy state, into the selective dissolving means to directly excite the dissolved or solution phase analyte molecules to an excited energy state;
directing the radiant energy emitted from the analyte molecules from the selective dissolving means to a detection means for measuring the emitted radiant energy;
measuring the emitted radiant energy; and
indicating the presence of the analyte.
0 Assignments
0 Petitions
Accused Products
Abstract
A fiber-optic sensor or analytical apparatus and associated method which utilizes the combination of (a) a solid, semi-solid or liquid sensor body which is capable of selectively dissolving the analyte compound to be detected and analyzed into the sensor body from the mixture or environment in which the compound is present and excluding undesired or interfering components, (b) a radiation source for directly radiating the analyte compound molecules to excite same to an elevated energy state, thereby causing the analyte compound itself to luminesce while dissolved in the sensor body, and (c) a radiation detector for detecting and measuring the light emitted from the excited analyte compound molecules when they return to a lower energy state. The selective dissolving sensor body provides a controlled environment in which both the source light directly excites the analyte molecules and the excited molecules emit light which is detected and measured. This invention thereby provides dual selectivity, first by selectively dissolving the analyte component into the sensor body and second by the direct selective/specific excitation of the analyte compound to cause it to specifically luminesce in a manner known to be characteristic of that compound. Improved sensitivity also is provided by this invention by the direct measurement relationship, i.e., the higher the concentration of the analyte compound, the greater the luminescent signal from the excited analyte compound.
-
Citations
11 Claims
-
1. A method for indicating the presence of an analyte component in a fluid mixture containing the analyte comprising:
-
contacting a selective dissolving means with the fluid mixture to dissolve analyte molecules from the fluid mixture, to provide the analyte molecules in the dissolved or solution phase in the selective dissolving means, and to provide a controlled environment in which the analyte molecules are exposed to a source radiant energy to directly excite the analyte molecules and raise the energy level of the analyte molecules to an excited energy state and a controlled environment in which the excited analyte molecules return to a lower energy level and emit radiant energy from the analyte molecules, said selective dissolving means being substantially transparent to the source radiant energy and to the emitted radiant energy; directing source radiant energy, which is suitable for directly exciting the analyte molecules to an elevated energy state, into the selective dissolving means to directly excite the dissolved or solution phase analyte molecules to an excited energy state; directing the radiant energy emitted from the analyte molecules from the selective dissolving means to a detection means for measuring the emitted radiant energy; measuring the emitted radiant energy; and indicating the presence of the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
Specification