Method for the simultaneous assay of ligands
First Claim
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1. A proximity assay method for simultaneously assaying two or more different and radioactively labeled ligands, comprising:
- incubating in a single assay container a sample containing two or more different and radioactively labeled ligands with two or more scintillative support materials, wherein said radioactively labeled ligands are labeled with a low energy isotope and each of said two or more scintillative support materials have different scintillation characteristics and each of said two or more scintillative support materials have attached to it biomolecules which specifically bind one of said two or more ligands, such than any of said two or more different and radioactively labeled ligands present in said sample will bind to each of said two or more scintillative support materials, and wherein the scintillative support materials are in close enough proximity to any bound radioactively labeled ligands to produce scintillations and are too far from any unbound radioactively labeled ligands to produce scintillations;
measuring the scintillation light output contribution produced by each of said two or more scintillative support materials; and
based on said scintillation light output contributions, determining the amount of each of the two or more different ligands in the sample.
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Abstract
The objective of the present invention is a method for determining two or more radioactively labeled ligands simultaneously in the same sample. According to the invention the ligands are determined with proximity assay so that for determining said ligands support materials with different scintillation characteristics are employed, each said support material having attached onto it molecules specifically binding one of said ligands. The support material consists totally or partially of scintillator.
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6 Claims
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1. A proximity assay method for simultaneously assaying two or more different and radioactively labeled ligands, comprising:
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incubating in a single assay container a sample containing two or more different and radioactively labeled ligands with two or more scintillative support materials, wherein said radioactively labeled ligands are labeled with a low energy isotope and each of said two or more scintillative support materials have different scintillation characteristics and each of said two or more scintillative support materials have attached to it biomolecules which specifically bind one of said two or more ligands, such than any of said two or more different and radioactively labeled ligands present in said sample will bind to each of said two or more scintillative support materials, and wherein the scintillative support materials are in close enough proximity to any bound radioactively labeled ligands to produce scintillations and are too far from any unbound radioactively labeled ligands to produce scintillations; measuring the scintillation light output contribution produced by each of said two or more scintillative support materials; and based on said scintillation light output contributions, determining the amount of each of the two or more different ligands in the sample. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification