Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
First Claim
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1. A method for making a fiber optic sensor able to detect at least one analyte of interest in a fluid sample, said method comprising the steps of:
- obtaining a preformed, unitary fluid optic array comprising a plurality of individually clad, fiber optic strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete optic array ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy;
preparing a photopolymerizable dye mixture comprising a light energy absorbing dye and a monomer mixture polymerizable by light energy;
placing one of said discrete optic array surfaces into contact with said photopolymerizable dye mixture;
introducing light energy to a first portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a first group of fiber optical strands within said preformed, unitary fiber optic array conveys and introduced light energy to a first spatial position on said contacted discrete optic array surface and causes said photopolymerizable dye mixture to photopolymerize at said first spatial position on said contacted discrete optic array surface as a first uninterrupted deposit of dye upon multiple strand end faces, said first spatially positioned uninterrupted deposit of dye reacting with one analyte of interest is a fluid sample; and
introducing light energy to a second portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a second group of fiber optical strands within said preformed, unitary fiber optic array conveys said introduced light energy to a second spatial position on said contacted discrete optic array surface and causes said photopolymerizable dye mixture to photopolymerize at said second uninterrupted deposit of dye upon multiple strand end faces, said second spatially positioned uninterrupted deposit of dye reacting with one analyte of interest in a fluid sample.
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Abstract
The present invention provides a unique fiber optic sensor which is able to conduct multiple assays and analyses concurrently using a plurality of different dyes immobilized at individual spatial positions on the surface of the sensor. The present invention also provides apparatus for making precise optical determinations and measurements for multiple analytes of interest concurrently and provides methods of detection for multiple analytes of interest which can be correlated with specific parameters or other ligands for specific applications and purposes.
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6 Claims
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1. A method for making a fiber optic sensor able to detect at least one analyte of interest in a fluid sample, said method comprising the steps of:
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obtaining a preformed, unitary fluid optic array comprising a plurality of individually clad, fiber optic strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete optic array ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy; preparing a photopolymerizable dye mixture comprising a light energy absorbing dye and a monomer mixture polymerizable by light energy; placing one of said discrete optic array surfaces into contact with said photopolymerizable dye mixture; introducing light energy to a first portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a first group of fiber optical strands within said preformed, unitary fiber optic array conveys and introduced light energy to a first spatial position on said contacted discrete optic array surface and causes said photopolymerizable dye mixture to photopolymerize at said first spatial position on said contacted discrete optic array surface as a first uninterrupted deposit of dye upon multiple strand end faces, said first spatially positioned uninterrupted deposit of dye reacting with one analyte of interest is a fluid sample; and introducing light energy to a second portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a second group of fiber optical strands within said preformed, unitary fiber optic array conveys said introduced light energy to a second spatial position on said contacted discrete optic array surface and causes said photopolymerizable dye mixture to photopolymerize at said second uninterrupted deposit of dye upon multiple strand end faces, said second spatially positioned uninterrupted deposit of dye reacting with one analyte of interest in a fluid sample. - View Dependent Claims (3, 4, 5, 6)
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2. A method for making a fiber optic sensor able to concurrently detect multiple analytes of interest in a fluid sample, said method comprising the steps of:
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obtaining a preformed, unitary fiber optic array comprising a plurality of individually clad, fiber optic strands disposed co-axially along their lengths and having two discrete optic array ends each of which is formed of multiple strand end faces, said preformed, unitary fiber optic array being of determinable configuration and dimensions, said two discrete optic array ends of said preformed, unitary fiber optic array presenting two discrete optic array surfaces for introduction and conveyance of light energy; preparing a first photopolymerizable dye mixture comprising a first light energy absorbing dye and a monomer mixture polymerizable by light energy; preparing a second photopolymerizable dye mixture comprising a second light energy absorbing dye and a monomer mixture polymerizable by light energy; placing one of said discrete optic array surfaces into contact with said first photopolymerizable dye mixture; introducing light energy to a first portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a first group of fiber optical strands within said preformed, unitary fiber optic array conveys said introduced light energy to a first spatial position on said contacted discrete optic array surface and causes said first photopolymerizable dye mixture to photopolymerize at said first spatial position on said contacted discrete optic array surface as a first uninterrupted deposit of dye upon multiple strand end faces, said spatially positioned first uninterrupted deposit of dye reacting with one analyte of interest in a fluid sample; placing said contacted discrete optic array surface of said preformed, unitary fiber optic array into additional contact with said second photopolymerizable dye mixture; and introducing light energy to a second portion of the other discrete optic array surface of said preformed, unitary fiber optic array such that a second group of fiber optical strands within said preformed, unitary fiber optic array conveys said introduced light energy to a second spatial position on said contacted discrete optic array surface and causes said second photopolymerizable dye mixture to photopolymerize at said second spatial position on said contacted discrete optic array surface as an second interrupted deposit of dye upon multiple strand end faces, said spatially positioned second uninterrupted deposit of dye reacting with one analyte of interest in a fluid sample.
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Specification