Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles
First Claim
Patent Images
1. An indicator reagent, useful for determining the presence or amount of an analyte in a test sample, comprising:
- a. an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol), polyacetylene and derivatives thereof, said particle having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared, andb. a specific binding member directly or indirectly attached to said particle.
1 Assignment
0 Petitions
Accused Products
Abstract
An indicator reagent, assay method and test kit for determining the presence or amount of an analyte in a test sample, in which the indicator reagent is formed by attaching an organic polymer latex particle, preferably a colored particle, to a specific binding member. The specific binding member can be adsorbed onto or covalently bound to the organic polymer latex particles. The organic polymer latex particles are readily detected by direct visual observation or can be detected and/or measured by appropriate instrumentation.
-
Citations
40 Claims
-
1. An indicator reagent, useful for determining the presence or amount of an analyte in a test sample, comprising:
-
a. an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol), polyacetylene and derivatives thereof, said particle having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared, and b. a specific binding member directly or indirectly attached to said particle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
-
14. A method for determining the presence or amount of an analyte in a test sample, comprising:
-
a. contacting the test sample sequentially or simultaneously with an indicator reagent and a capture reagent, directly or indirectly attached to a specific binding member, said indicator reagent comprising an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared; and b. allowing said indicator reagent to bind to the analyte in the test sample or to said capture reagent; c. detecting said indicator reagent; and d. determining thereby the presence or amount of analyte in the test sample. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
-
-
40. A test kit for determining the presence or amount of an analyte in a test sample, comprising:
-
a. an indicator reagent comprising an organic polymer latex particle prepared from the polymerization of a plurality of nonchromophoric monomers, said particle selected from the group consisting of poly(pyrrole), polyphenylene, poly(aniline), poly(thiophene), poly(naphthalene), poly(thiophenol) polyacetylene and derivatives thereof, and having light absorbance characteristics resulting from a conjugated structure from the polymerization of said monomers wherein said polymer latex particle exhibits increased absorbance in the visible spectrum compared to the absorbance in the visible spectrum of the aggregate of nonchromophoric monomers from which it is prepared, said indicator reagent being directly or indirectly attached to a specific binding member and capable of producing a detectable signal indicative of the presence or amount of an analyte in a test sample; and b. one or more assay components including capture reagents, ancillary binding members, solid phase material, buffers, water, stabilizers, surfactants, standards and calibrators.
-
Specification