Process for producing a transglutaminase derived from streptomyces

• US 5,252,469 A
• Filed: 08/26/1991
• Issued: 10/12/1993
• Est. Priority Date: 08/27/1990
• Status: Expired due to Term

• Current Assignee: Amano Pharmaceutical Co., Ltd.
• Original Assignee: Amano Pharmaceutical Co., Ltd.
• Inventors: Andou, Hiroyasu, Matsuura, Akira, Hirose, Susumu
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): Weber, Jon P.
• Application Number:
  US07/750,160
• Time in Patent Office: 778 Days
• Field of Search: 435/227, 435/193, 435/71.2
• US Class Current: 435/71.2
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 9/1044 Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
• CPC Class Codes: C12R 2001/56 Streptomyces lavendulae

Abstract

A process for producing a transglutaminase which comprises (1) culturing a microorganism having the identifying characteristics of a microorganism selected from the group consisting of Streptomyces sp. No. 83, deposited as FERM BP-3505, Streptomyces lavendulae No. 466, deposited as FERM BP-3506, and mutants thereof, wherein the microorganism is capable of producing a transglutaminase, wherein the transglutaminase is an enzyme which catalyzes an acyl transfer reaction of a γ-carboxyamide group of a glutamine residue in a peptide or protein chain in the presence or absence of Ca⁺⁺ and wherein the transglutaminase has an isoelectric point of 10 or 6.8, respectively, and is inhibited by Pb ions, and (2) recovering the transglutaminase from the culture obtained is disclosed.

Claims

1. A process for producing a transglutaminase comprising the steps of:

(1) culturing a microorganism having all of the identifying characteristics of the microorganism, Streptomyces sp. No. 83, deposited as FERM BP-3505, and mutants thereof, which produces a transglutaminase; and

(2) recovering said transglutaminase from the culture obtained,

wherein said transglutaminase is an enzyme which catalyzes an acyl transfer reaction of a γ

-carboxyamide group of a glutamine residue in a peptide or protein chain in the presence or absence of Ca⁺⁺, and wherein said transglutaminase has an isoelectric point of 10, and is inhibited by Pb ions.

3. The process for producing a transglutaminase as claimed in claim 1, wherein said microorganism is Streptomyces sp. No. 83, deposited as FERM BP-3505.

4. The process for producing a transglutaminase as claimed in claim 1, wherein said microorganism is Streptomyces lavendulae No. 466, deposited as FERM BP-3506.

2. A process for producing a transglutaminase comprising the steps of:

(1) culturing a microorganism having all of the identifying characteristics of the microorganism, Streptomyces lavendulae No. 466, deposited as FERM BP-3506, and mutants thereof, which produces a transglutaminase; and

(2) recovering said transglutaminase from the culture obtained,

wherein said transglutaminase is an enzyme which catalyzes an acyl transfer reaction of a γ

- carboxyamide group of a glutamine rersidue in a peptide or protein chain in the presence or absence of Ca⁺⁺, and wherein said transglutaminase has an isoelectric point of 6.8, and is inhibited by Pb ions.