Promoters for use in aspergillus

• US 5,252,726 A
• Filed: 03/23/1992
• Issued: 10/12/1993
• Est. Priority Date: 09/04/1987
• Status: Expired due to Term

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novo Nordisk A/S
• Inventors: Woldike, Helle F.
• Primary Examiner(s): Schwartz, Richard A.
• Assistant Examiner(s): CARTER, PHILIP
• Application Number:
  US07/859,596
• Time in Patent Office: 568 Days
• Field of Search: 536/27, 435/69.1, 435/172.3, 435/203, 435/254, 435/201
• US Class Current: 536/24.1
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C12N 15/80 for fungi
• CPC Class Codes: C12N 9/242 Fungal source
• CPC Class Codes: C12N 9/62 from Aspergillus

Abstract

A process for expression of a protein product in Aspergillus is disclosed. The process comprises transforming an Aspergillus strain with a vector system comprising DNA-sequences encoding a promoter including upstream activating sequences derived from an A. niger amylase, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in Aspergillus, preferably A. niger. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus, preferably Aspergillus niger being derived from a gene encoding an A. niger amylase. The A. niger amylases are the neutral and acid stable α-amylases and a new amylase not so far described and designated XA amylase. Also disclosed is the novel amylase from A. niger XA amylase.

Claims

1. A promoter and upstream activating sequence derived from an Aspergillus niger neutral alpha-amylase gene having the following sequence. ##STR7## or a fragment thereof having substantially the same promoter activity as said sequence.

2. A promoter and upstream activating sequence derived from an Aspergillus niger neutral alpha-amylase gene having the following sequence ##STR8## or a fragment thereof having substantially the same promoter activity as said sequence.