Method of immobilizing biofunctional material, and element prepared thereby, and measurement by using the same element
First Claim
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1. A microbioelectrode produced by direct immobilization of a biologically active substance on a surface of an electrically conductive material which comprises the steps of:
- (a) depositing fine particles of noble metal or a compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black on the surface of an electroconductive material so as to form a porous conductive material or a conductive fine particle layer on said surface;
(b) immersing the resulting porous conductive material or conductive fine particle layer in a solution containing an active substance so as to impregnate the active substance deeply into the conductive porous material or conductive fine particle layer; and
(c) treating said porous conductive material or conductive fine particle layer by anodic oxidation.
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Abstract
A microbioelectrode having high performance produced by immobilization by incorporating a biologically active substance in the interior or on the surface of a porous conductive material layer or fine particle layer which has been deposited on the surface of a transducing material. The inventive microbioelectrode demonstrates sufficient output even if the sensor using the electrode is very small in size.
11 Citations
20 Claims
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1. A microbioelectrode produced by direct immobilization of a biologically active substance on a surface of an electrically conductive material which comprises the steps of:
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(a) depositing fine particles of noble metal or a compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black on the surface of an electroconductive material so as to form a porous conductive material or a conductive fine particle layer on said surface; (b) immersing the resulting porous conductive material or conductive fine particle layer in a solution containing an active substance so as to impregnate the active substance deeply into the conductive porous material or conductive fine particle layer; and (c) treating said porous conductive material or conductive fine particle layer by anodic oxidation. - View Dependent Claims (2, 3, 4, 14, 15)
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5. A biologically active substance immobilized microbioelectrode comprising a fine particle electrically conductive surface layer incorporating an immobilized biologically active substance therein, formed by the steps of:
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(a) depositing fine particles of noble metal or a compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black on a surface of an electroconductive material so as to form a porous conductive material or a conductive fine particle layer on said surface; (b) immersing the resulting porous conductive material or conductive fine particle layer in a solution containing said active substance so as to impregnate said active substance deeply into the conductive porous material or conductive fine particle layer; and (e) treating said porous conductive material or conductive fine particle layer by anodic oxidation. - View Dependent Claims (6, 7, 8, 16, 17)
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9. A method for immobilizing a biologically active substance within an electrically conductive material which comprises the steps of:
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(a) depositing fine particles of noble metal or a compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black on a surface of an electroconductive material so as to form a porous conductive material or a conductive fine particle layer on said surface; (b) immersing the resulting porous conductive material or conductive fine particle layer in a solution containing said active substance so as to impregnate said active substance deeply into the conductive porous material; and (c) treating said porous conductive material or conductive fine particle layer by anodic oxidation. - View Dependent Claims (10, 11, 12)
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13. A microbioelectrode designed to have biologically active substances immobilized in the fine pores or on the surface of a porous conductive material or conductive fine particle layer which is deposited on the surface of a transducing electrode, wherein the porous conductive material or conductive fine particle layer consists of finely divided particles of noble metal or a compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black, thereby having biological and electrochemical reactions occur simultaneously so as to enable direct transduction into an electric signal.
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18. A microbioelectrode produced by direct immobilization of a biologically active substance within a porous layer of precious metal material or compound found on a conductive substrate, which comprises the steps of:
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(a) depositing porous precious metal material or compound selected from the group consisting of platinum black, gold black, particulate rhodium oxide, palladium black and iridium black, on the surface of an electroconductive substrate, so as to form a layer of the porous precious metal material or compound of said surface; (b) immersing the resulting layer on said surface in a solution containing said biologically active substance so as to impregnate said active substance deeply in said layer; and (c) treating said layer by anodic oxidation. - View Dependent Claims (19, 20)
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Specification